Difference between revisions of "Strifler 2016 PLOS ONE"
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|title=Strifler G, Tuboly E, Szél E, Kaszonyi E, Cao C, Kaszaki J, Mészáros A, Boros M, Hartmann P (2016) Inhaled methane limits the mitochondrial electron transport chain dysfunction during experimental liver ischemia-reperfusion injury. PLOS ONE 11:e0146363. | |title=Strifler G, Tuboly E, Szél E, Kaszonyi E, Cao C, Kaszaki J, Mészáros A, Boros M, Hartmann P (2016) Inhaled methane limits the mitochondrial electron transport chain dysfunction during experimental liver ischemia-reperfusion injury. PLOS ONE 11:e0146363. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26741361 PMID: 26741361 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26741361 PMID: 26741361 Open Access] | ||
|authors=Strifler G, Tuboly E, Szel E, Kaszonyi E, Cao C, Kaszaki J, Meszaros A, Boros M, Hartmann P | |authors=Strifler G, Tuboly E, Szel E, Kaszonyi E, Cao C, Kaszaki J, Meszaros A, Boros M, Hartmann P | ||
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|enzymes=Complex IV;cytochrome c oxidase | |enzymes=Complex IV;cytochrome c oxidase | ||
|injuries=Ischemia-reperfusion | |injuries=Ischemia-reperfusion | ||
|couplingstates=LEAK | |couplingstates=LEAK, OXPHOS | ||
|substratestates=CI, CII, CIV, CI&II, ROX | |substratestates=CI, CII, CIV, CI&II, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2016-02 | ||
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Revision as of 15:23, 3 March 2016
Strifler G, Tuboly E, Szél E, Kaszonyi E, Cao C, Kaszaki J, Mészáros A, Boros M, Hartmann P (2016) Inhaled methane limits the mitochondrial electron transport chain dysfunction during experimental liver ischemia-reperfusion injury. PLOS ONE 11:e0146363. |
Strifler G, Tuboly E, Szel E, Kaszonyi E, Cao C, Kaszaki J, Meszaros A, Boros M, Hartmann P (2016) PLOS ONE
Abstract: Methanogenesis can indicate the fermentation activity of the gastrointestinal anaerobic flora. Methane also has a demonstrated anti-inflammatory potential. We hypothesized that enriched methane inhalation can influence the respiratory activity of the liver mitochondria after an ischemia-reperfusion (IR) challenge.
The activity of oxidative phosphorylation system complexes was determined after in vitro methane treatment of intact liver mitochondria. Anesthetized Sprague-Dawley rats subjected to standardized 60-min warm hepatic ischemia inhaled normoxic air (n = 6) or normoxic air containing 2.2% methane, from 50 min of ischemia and throughout the 60-min reperfusion period (n = 6). Measurement data were compared with those on sham-operated animals (n = 6 each). Liver biopsy samples were subjected to high-resolution respirometry; whole-blood superoxide and hydrogen peroxide production was measured; hepatocyte apoptosis was detected with TUNEL staining and in vivo fluorescence laser scanning microscopy.
Significantly decreased complex II-linked basal respiration was found in the normoxic IR group at 55 min of ischemia and a lower respiratory capacity (~60%) and after 5 min of reperfusion. Methane inhalation preserved the maximal respiratory capacity at 55 min of ischemia and significantly improved the basal respiration during the first 30 min of reperfusion. The IR-induced cytochrome c activity, reactive oxygen species (ROS) production and hepatocyte apoptosis were also significantly reduced.
The normoxic IR injury was accompanied by significant functional damage of the inner mitochondrial membrane, increased cytochrome c activity, enhanced ROS production and apoptosis. An elevated methane intake confers significant protection against mitochondrial dysfunction and reduces the oxidative damage of the hepatocytes.
• O2k-Network Lab: HU Szeged Boros M
Labels: MiParea: Respiration, mt-Medicine, Pharmacology;toxicology
Stress:Ischemia-reperfusion Organism: Rat Tissue;cell: Liver Preparation: Homogenate, Isolated mitochondria Enzyme: Complex IV;cytochrome c oxidase
Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k
2016-02