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| ==Bioblast alert== | | ==Bioblast alert== |
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| ===Bioblast alert - in preparation===
| | Moved to [[Bioblast alert]] and [[Talk:Bioblast alert]] |
| ====Bioblast alert #02====
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| *'''[http://www.oroboros.at/index.php?id=ioc64 O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India]''': 2011-12-08.
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| *O2k-Publication: '''Heart failure and mitochondrial dysfunction'''. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
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| ::[[Lemieux 2011 IJBCB|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int. J. Biochem. Cell Biol. 43: 1729–1738]]. -- [[Talk:Lemieux 2011 IJBCB|Discussion: Complementary results on the PGC-1a pathway]].
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| *'''[[Scientific service]]''': '''[[Tissue storage]]''' on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with [[SUIT protocol]]s.
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| *Term of the week: '''[[Uncoupled]], [[non-coupled]], [[dyscoupled]]''' - a hallmark of high-resolution respirometry is a matching '''high-resolution terminology'''.
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| ::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[non-coupled|uncoupled]]''' respiration''.
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| ====Bioblast alert ## to select====
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| *O2k-Publication: '''Protocols for HRR: from intact cells to permeabilized muscle fibres'''
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| ::[[Pesta 2012 MethodsMolBiol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol. Biol. 810: 25-58.]]
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| ====Bioblast alert ## to select====
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| *O2k-Publication: '''Fluorescence in the O2k'''
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| ::[[Hickey 2011 JCPB|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print] ]]
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| ::[[Anderson 2011 AJP|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am. J. Physiol. Heart Circ. Physiol. 300: H118-124.]]
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| *MiPNet Reference Laboratory: [[NZ Auckland Hickey AJ]] - 6 O2k, guest lecturer at IOC66, joint project (Marsden), guest tutor at IOC59.
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| ====Bioblast alert ## to select====
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| *O2k-Publication: ''Efficiency, basal respiration, and oxygen kinetics in human muscle'' or '''Mitochondrial efficiency and P/O flux ratios: new perspectives in mitochondrial physiology''' (see [[MitoCom_lectures#Journal_club|Journal club]]);
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| ::[[Larsen 2011 CellMetab|Larsen FJ, Schiffer TA, Borniquel S, Sahlin K, Ekblom B, Lundberg JO, Weitzberg E (2011) Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab. 13: 149-159]].
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| ::[[Larsen 2011 FASEBJ|Larsen FJ, Schiffer TA, Sahlin K, Ekblom B, Weitzberg E, Lundberg JO (2011) Mitochondrial oxygen affinity predicts basal metabolic rate in humans. FASEB J. 25: 2843-2852]].
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| *Before alert, ask to join as MiPNet Reference Lab, and present new MiPNet Reference Lab
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| *Term: efficiency versus P/O ratio
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| ===Bioblast alert - previous===
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| ====Bioblast alert #01 (2011-11-11)====
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| *'''[http://www.oroboros.at/index.php?ioc66 O2k-Fluorimetry Workshop IOC66]''': 2012-03-15 to 16. OROBOROS INSTRUMENTS, Innsbruck, Austria. The first series of the '''[[O2k-Fluorescence Module]]''' will be delivered in '''April 2012''', for orders received by end of January 2012.
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| *O2k-Publication: A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
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| ::[[Pesta_2011_AJP|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am. J. Physiol. Regul. Integr. Comp. Physiol. 301: R1078–R1087]].
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| *'''[[Scientific service]]''': [[ETS capacity]] plays a key role in such analyses, which cannot be achieved quantitatively without careful '''[[Optimum uncoupler concentration|uncoupler titrations]].
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| *Term of the month: '''[[Basal respiration]]''' - a hallmark of high-resolution respirometry is a matching '''high-resolution terminology'''.
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| ==Bioblast accessed== | | ==Bioblast accessed== |
