Talk:Bioblast alert 2015: Difference between revisions
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* Scientific service: Oxygen regime for permeabilized fibres vs. isolated mitochondria - reoxygenations in MiR06. | * Scientific service: Oxygen regime for permeabilized fibres vs. isolated mitochondria - reoxygenations in MiR06. | ||
* Various chemicals: | * Various chemicals: | ||
: Carboxyatractyloside vs. atractyloside | |||
:: | |||
Gnaiger E | :: Gnaiger E: When we used atractyloside (Atr), carboxyatractyloside (Cat) was off the market, hence we had no other choice. Cat is available again. Even if it costs more, it is worth more, and with Cat available, I would not bother to find out more about Atr. | ||
When we used atractyloside (Atr), carboxyatractyloside (Cat) was off the market, hence we had no other choice. Cat is available again. Even if it costs more, it is worth more, and with Cat available, I would not bother to find out more about Atr | |||
:: Chinopoulos C: I have used the Sigma Cat as well, but not for titration studies. It works perfectly at 1-2 µM concentrations, if you want to block completely all ANTs. The MW is 770.8. | |||
The Sigma Cat originates from Atr extracted from Xanthicum sibericum. A carboxylation is a further chemical modification. The Merck Cat originates from Atr from Atractylis gummifera, which is identical to that from Xanthicum. A carboxylation is a further chemical modification. Because of this modification which is man-made, there may be different potencies. For titration studies (0-200 nM Cat), the one from Merck (Atractylis gummifera) is strongly recommended. - Christos (2012-04-19) | The Sigma Cat originates from Atr extracted from Xanthicum sibericum. A carboxylation is a further chemical modification. The Merck Cat originates from Atr from Atractylis gummifera, which is identical to that from Xanthicum. A carboxylation is a further chemical modification. Because of this modification which is man-made, there may be different potencies. For titration studies (0-200 nM Cat), the one from Merck (Atractylis gummifera) is strongly recommended. - Christos (2012-04-19) | ||
Unfortunately Merck does not sell the Cat from Atractylis gummifera anymore and currently only offers the Cat extracted from Xanthicum sibericum (as does Sigma). - Michael (2012-04-20) | ::Chu MJJ: Unfortunately Merck does not sell the Cat from Atractylis gummifera anymore and currently only offers the Cat extracted from Xanthicum sibericum (as does Sigma). - Michael (2012-04-20) | ||
== Bioblast alert - concept == | == Bioblast alert - concept == | ||
Revision as of 15:29, 2 May 2012
Bioblast alert - in preparation
Bioblast alert 2012 (##): 2012-##-##
Mitochondrial dys/function in heart vs. brain:
- Wüst RC, Myers DS, Stones R, Benoist D, Robinson PA, Boyle JP, Peers C, White E, Rossiter HB (2011) Regional skeletal muscle remodeling and mitochondrial dysfunction in right ventricular heart failure. Am J Physiol Heart Circ Physiol. 302: H402-11. Wuest 2012 Am J Physiol Heart Circ Physiol.
- Boyle JP, Hettiarachchi NT, Wilkinson JA, Pearson HA, Scragg JL, Lendon C, Al-Owais MM, Kim CB, Myers DM, Warburton P, Peers C (2012) Cellular consequences of the expression of Alzheimer's disease-causing presenilin 1 mutations in human neuroblastoma (SH-SY5Y) cells. Brain Res Epub ahead of print. Boyle 2012 Brain Res.
MiPNet Laboratory of the week
Term of the week
Bioblast alert ### to select
- Scientific service: Oxygen regime for permeabilized fibres vs. isolated mitochondria - reoxygenations in MiR06.
- Various chemicals:
- Carboxyatractyloside vs. atractyloside
- Gnaiger E: When we used atractyloside (Atr), carboxyatractyloside (Cat) was off the market, hence we had no other choice. Cat is available again. Even if it costs more, it is worth more, and with Cat available, I would not bother to find out more about Atr.
- Chinopoulos C: I have used the Sigma Cat as well, but not for titration studies. It works perfectly at 1-2 µM concentrations, if you want to block completely all ANTs. The MW is 770.8.
The Sigma Cat originates from Atr extracted from Xanthicum sibericum. A carboxylation is a further chemical modification. The Merck Cat originates from Atr from Atractylis gummifera, which is identical to that from Xanthicum. A carboxylation is a further chemical modification. Because of this modification which is man-made, there may be different potencies. For titration studies (0-200 nM Cat), the one from Merck (Atractylis gummifera) is strongly recommended. - Christos (2012-04-19)
- Chu MJJ: Unfortunately Merck does not sell the Cat from Atractylis gummifera anymore and currently only offers the Cat extracted from Xanthicum sibericum (as does Sigma). - Michael (2012-04-20)
Bioblast alert - concept
Here is a brief summary on the concept of Bioblast alert.
Some of the following topics (not more than four per alert) will be selected for a Bioblast alert.
MiPNet O2k-Publications
- Topic: O2-kinetics; coupling; pathology; ...
- Preparation: Homogenate/isolated mitochondria; intact/permeabilized cells; permeabilized fibre/in vivo
- Protocols
MiPNet Laboratories of the World
Contact the MiPNet laboratories: show link on the wiki, ask for updates on group, references, website, perhaps major projects (HypoxiaNet), perhaps add pictures, perhaps keywords/short description. Circular: link to presented lab / special occasion (e.g. publication; conference presentation; new member; PhD thesis, ..
High-resolution terminology
- high-resolution terminology - matching measurement at high-resolution. Term of the week: Basal respiration
- Define – Measure: terminology and experimental approach
- Define – Clarify: consider discrepancies, resolve problems
MiPevents
- Conferences and Workshops
Bioblast
- What is new or updated on the website: Lists (chemicals, protocols, scientific service, ..)
- Projects in mitochondrial science (COST HypoxiaNet; Framework programs; ..)
- Links on the Bioblast website; networking in mitochondrial science, presentation of: MIG List, Mitochondrion journal and website; Mitochondrial Societies
- MitoCom News
Bioblast editors
A Bioblast editor may select an O2k-Publication (or a small number of related publications), particularly from a MiPNet Reference Laboratory, may get in contact with the particular reference lab, update info on the lab, and ‘present’ the lab in the alert. A term may be selected (perhaps related to the publication), updated, discussed in our group, and then presented in the alert. An editor does not even have to present an entire alert, but may prepare individual ‘points’, which then can be added to a particular alert. As Bioblast is updated, the updated ‘article’ (term, publication etc) can be added to the prepared points for a future alert.
