Difference between revisions of "Talk:MiR06"
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==Cost== | |||
''' | '''Q''': For calculating the running costs: what are the typical costs for MiR05 per run? | ||
For a general discussion of running costs see [http://www.oroboros.at/index.php?low-o2k-running-costs | |||
'''A''': For a general discussion of running costs see [http://www.oroboros.at/index.php?low-o2k-running-costs 'Low O2k running costs']. | |||
We once calculated the chemical costs for MiR05 to be about 60 € /liter. | We once calculated the chemical costs for MiR05 to be about 60 € /liter. | ||
Calculated with 3 ml/per chamber (for a 2 ml chamber) this would amount to about 0,2 € per chamber and run. | Calculated with 3 ml/per chamber (for a 2 ml chamber) this would amount to about 0,2 € per chamber and run. | ||
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[[User:Fasching Mario|Fasching Mario]] 12:07, 13 October 2011 (CEST) | [[User:Fasching Mario|Fasching Mario]] 12:07, 13 October 2011 (CEST) | ||
==Inhibitory effects== | |||
*Lack of ADP stimulation with either glutamate+malate or succinate+rotenone excludes an explanation by rotenone retention effects. | |||
*If you do not experience any problem using intact cells in a protocol with [[ROUTINE]] respiration, [[LEAK]] respiration, and [[ETS]] capacity, then the instrument works well and the ADP-stimulation problem is unrelated to a possible problem with the POS. | |||
*Do you observe the problems when using mitochondrial respiration medium (MiR05 or [[MiR06]] with permeabilized cells), but not when using cell culture medium (intact cells)? If yes, then there could be an inhibitory compound in MiR05/MiR06. In one lab, MiR05 had to prepare always fresh: After freezing, there was an inhibition or respiration. In general, MiR05, MiR06 or MiR07 is perfectly OK after freezing. There must have been something in the stock chemicals that caused an inhibitory effect after freezing. Perhaps you can get a batch of medium form another lab, just to try if this medium might solve the problem? | |||
--[[User:Gnaiger Erich|Gnaiger Erich]] 20:00, 8 November 2011 (CET) | |||
Revision as of 21:00, 8 November 2011
Cost
Q: For calculating the running costs: what are the typical costs for MiR05 per run?
A: For a general discussion of running costs see 'Low O2k running costs'.
We once calculated the chemical costs for MiR05 to be about 60 € /liter.
Calculated with 3 ml/per chamber (for a 2 ml chamber) this would amount to about 0,2 € per chamber and run.
There are some caveats: calculation based on Austrian prices; excluding VAT, no "left overs" taken into acount,ie, based on the assumption you use the entire package, no work, no water, no KOH for adjusting the pH.....
Fasching Mario 12:07, 13 October 2011 (CEST)
Inhibitory effects
- Lack of ADP stimulation with either glutamate+malate or succinate+rotenone excludes an explanation by rotenone retention effects.
- If you do not experience any problem using intact cells in a protocol with ROUTINE respiration, LEAK respiration, and ETS capacity, then the instrument works well and the ADP-stimulation problem is unrelated to a possible problem with the POS.
- Do you observe the problems when using mitochondrial respiration medium (MiR05 or MiR06 with permeabilized cells), but not when using cell culture medium (intact cells)? If yes, then there could be an inhibitory compound in MiR05/MiR06. In one lab, MiR05 had to prepare always fresh: After freezing, there was an inhibition or respiration. In general, MiR05, MiR06 or MiR07 is perfectly OK after freezing. There must have been something in the stock chemicals that caused an inhibitory effect after freezing. Perhaps you can get a batch of medium form another lab, just to try if this medium might solve the problem?
--Gnaiger Erich 20:00, 8 November 2011 (CET)
