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| | * This page has been moved to http://intern.oroboros.at/index.php/Library_of_Protocols |
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| == Future Projects/Protocols ==
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| === Maus Herz - Shredder vs. fibres ===
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| * November experimental days: 2+3, 9+10, 16+17
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| * '''Protocol 5''': G + M + D + c + P + D + S + D + FCCP + Rot + Mna + Ama
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| === Fish - Oxidative stress ===
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| * Protocol 1: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + Omy + FCCP+ Rot + Mna + Ama | |
| To reduce the number of protocols, I suggest to skip Protocol 1, and try Protocol 2 only (Protocol 1 without Omy was applied before, the results will serve as a basis to describe the general substrate control pattern).
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| * Protocol 2: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S * Rot + Omy + FCCP + + Rot + Mna + Ama
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| ''Correction (EG, 2011-10-11)'':
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| * '''Protocol 2''': G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + D + Rot + Omy + FCCP + Mna + Ama
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| : see the following protocol for the H202 - oxidative stress - treatment: [[DSL_intern#Protocol:_2011-10-06]]
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| : '''Rational:''' calculation of P/E is not possible, but the effect of Omy is of interest
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| === Mouse brain - Shredder ===
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| * Idea by Erich, 2011-10-07
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| * Method: Shredder
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| * Rational: test these different protocols to see the effect of cytochrome c and Oligomycin at different positions in the protocol
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| * Protocol 1: PGM + '''c + D-titration + Omy''' + F-titration + Rot + Mna + Ama
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| * Protocol 2: PGM + '''D-titration + c + Omy''' + F-titration + Rot + Mna + Ama
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| * Protocol 3: PGM + '''D-titration + Omy + c''' + F-titration + Rot + Mna + Ama
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