Template:BioblastAlert2021

Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21

A quote from MitoPathways Chapter 3. Normalization:

Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmol∙s^-1∙mg^-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmol∙s^-1∙mg^-1 in platelets and senescent fibroblasts and 40 to 80 pmol∙s^-1∙mg^-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ― Are these values comparable? - »Gnaiger 2020 BEC MitoPathways«.

Statistical evaluation on replica in high-resolution respirometry with permeabilized skeletal muscle fibers

from the O2k-Network »AU Melbourne Bishop DJ«

Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - »Bioblast link«

Effect of temperature on mitochondrial respiratory capacity of cardiac permeabilized fibers

from the O2k-Network »AT Innsbruck Gnaiger E« , »CA Rimouski Blier PU« and »CA Edmonton Lemieux H«

Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. »Bioblast link«

What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?

For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- »Optimum ADP concentration in pfi«

Bioblast alert 2021(01) - MitoPathways: the new BEC edition of the Blue Book: 2021-01-05

The 5^th edition of MitoPathways features new chapters on additivity of mitochondrial pathway capacities and protonmotive pressure – one step beyond ∆Ψ and the protonmotive force.

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2: 112pp. doi:10.26124/bec:2020-0002. - »Open Access«