Template:Chemical SUIT-033 NADH mt D081
From Bioblast
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1D.1 | ADP (D) | |
2PGM | Pyruvate (P), Glutamate (G), and Malate (M) | |
3D2.5 | ADP (D) | |
4Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
5Myx | Myxothiazol | We do not recommend the use of any other inhibitor of complex III, like Antimycin A (Ama), due to the chemical background effect on fluorescence. |
6Reox | Reoxygenation can be performed by opening the chamber, see: Open chamber. |