Template:Current week ecosystem agenda: Difference between revisions

Revision as of 12:50, 2 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 44
+Week 18
-*'''Oct- 30 Mo'''
+* '''Apr-29 Mo'''
-----
-*'''Oct- 31 Tu'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' How to interpret cytochrome ''c'' efficiency '''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-Stimulation of OXPHOS or ET capacity upon addition of cytochrome ''c''  provides evidence of cytochrome ''c'' loss, indicating damage of the mitochondrial outer membrane, which could be caused by sample preparation or pathological conditions. <br>
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-- »'''[https://wiki.oroboros.at/index.php/Cytochrome_c_control_efficiency Cytochrome ''c'' control efficiency]'''« <br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- communicated by »[[Schmitt Sabine]]« and »[[Leo Elettra]]«
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Nov- 01 We'''
+* '''Apr-30 Tu'''
 ----
-*'''Nov- 02 Th'''
+* '''May-01 We'''
 ----
-*'''Nov- 03 Fr'''
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"Peripheral impairments at the skeletal muscle level, namely reduced mitochondrial function and the presence of markers of impaired mitochondrial biogenesis emerged as major determinants of exercise intolerance and fatigue [in patients with post-acute sequelae of SARS-CoV-2 infection]"''' <br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-* Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. [[Colosio 2023 J Appl Physiol (1985)|»Bioblast link«]]
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
+* '''May-03 Fr'''
 '''<big>Next Week</big>'''
+Week 19
-Week 45
+* '''May-06 Mo'''
-*'''Nov- 06 Mo'''
-----
-*'''Nov- 07 Tu'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''How often should I re-assemble the O2k-chamber?'''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-We recommend disassembling, cleaning and re-assembling the O2k-chamber at least every 1-2 years. It is also necessary to disassemble it when there is persistent biological or protein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-<br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- »'''[https://wiki.oroboros.at/index.php/O2k-chamber#O2k-chamber_assembly_-_possible_problems O2k-chamber assembly - possible problems]'''« and »'''[https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 O2k-Videosupport: Insert O2k-chamber]'''«<br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Schmitt Sabine]]«
 |}
 ----
-*'''Nov- 08 We'''
+* '''May-07 Tu'''
 ----
-*'''Nov- 09 Th'''
+* '''May-08 We'''
 ----
-*'''Nov- 10 Fr'''
+* '''May-09 Th'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|''' When should I analyze my data as Flux control ratios (''FCR''s)?'''<br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-''FCR'' enables the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O<sub><small>2</small></sub> fluxes in different respiratory states to the maximum flux in a common reference state. <br>
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-- »'''[[Flux control ratio]]'''« <br>
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
 |}
-----
+* '''May-10 Fr'''