Template:Current week ecosystem agenda: Difference between revisions

Revision as of 12:50, 2 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 47
-*'''Nov- 20 Mo'''
+Week 18
+* '''Apr-29 Mo'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Do I need to change the filter set of the O2k-Fluo Smart-Module?'''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-Because two different filter sets can be used with the blue Fluo-Sensors, filters should be changed according to the fluorophore that will be used. Extra filters are provided for replacement in case of damage.
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-<br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- »'''[[MiPNet28.09_O2k-Fluo_Smart-Module_manual|O2k-Fluo Smart-Module manual]]'''« and »'''[[O2k-Fluo_Smart-Module#Fluorescence_dyes_and_settings|O2k-Fluo Smart-Module - Fluorescence dyes and settings]]'''« <br>
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
-- communicated by »[[Cardoso Luiza]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Nov- 21 Tu'''
+* '''Apr-30 Tu'''
 ----
-*'''Nov- 22 We'''
+* '''May-01 We'''
-:{|
-| width="100" align="center" |[[File:2023.4.jpg|right|150px|link=https://doi.org/10.26124/bec:2023-0004| Oliveira 2023 BEC]]
-|'''“The cautionary note provided by these studies emphasizes the need for careful consideration of which probes and conditions can be used when assessing mitochondrial physiology in ''Drosophila''.”''' <br>
-* Oliveira MF, Aveiro Y, Couto-Lima CA (2023) A navigation chart to avoid turbulence in ''Drosophila'' mitochondrial research. Bioenerg Commun 2023.4. https://doi.org/10.26124/bec:2023-0004
-:from the O2k-Network »[[BR Rio de Janeiro Oliveira MF]]«
-|}
 ----
-*'''Nov- 23 Th'''
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"Thoughtful design and execution of O2k experiments result in data that become a powerhouse for addressing many mitochondrial respiration research questions. "''' <br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-* Walsh MA, Musci RV, Jacobs RA, Hamilton KL (2023)  A practical perspective on how to develop, implement, execute, and reproduce high-resolution respirometry experiments: The physiologist's guide to an Oroboros O2k [[Walsh 2023 FASEB J|»Bioblast link«]]
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-:from the O2k-Network »[[US CO Colorado Springs Jacobs RA]]« and »[[US CO Fort Collins Hamilton K]]«
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Nov- 24 Fr'''
+* '''May-03 Fr'''
-----
 '''<big>Next Week</big>'''
-Week 48
+Week 19
-*'''Nov- 27 Mo'''
+* '''May-06 Mo'''
-----
-*'''Nov- 28 Tu'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Protocols that SUIT your research question'''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-Using '''S'''ubstrate-'''U'''ncoupler-'''I'''nhibitor-'''T'''itration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay. <br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-- »'''[https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocols]'''« and »'''[https://bioblast.at/index.php/SUITbrowser SUITbrowser]'''« <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
 - communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Nov- 29 We'''
+* '''May-07 Tu'''
 ----
-*'''Nov- 30 Th'''
+* '''May-08 We'''
 ----
-*'''Dec- 01 Fr'''  <span style="color:red">World AIDS day</span>
+* '''May-09 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"We therefore hypothesized that the susceptibility to HIV-1 infection of FOXO1-inhibited resting T cells could be due to an increased cell metabolism. (...) Results obtained by high-resolution respirometry experiments of [human peripheral blood T cells] PBT (...) showed that [ROUTINE] respiration at the steady state was increased by [FOXO1 inhibitor] AS1842856."''' <br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-* Roux A, Leroy H, De Muylder B, Bracq L, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G, Mangeney M (2019) FOXO1 transcription factor plays a key role in T cell-HIV-1 interaction.  [[Roux 2019 PLoS Pathog|»Bioblast link«]]
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-:from the O2k-Network »[[FR Paris Bouillaud F]]«
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
 |}
-----
+* '''May-10 Fr'''