Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 47
-*'''Nov- 20 Mo'''
+Week 21
+* '''May-20 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Do I need to change the filter set of the O2k-Fluo Smart-Module?'''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-Because two different filter sets can be used with the blue Fluo-Sensors, filters should be changed according to the fluorophore that will be used. Extra filters are provided for replacement in case of damage.
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-<br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- »'''[[MiPNet28.09_O2k-Fluo_Smart-Module_manual|O2k-Fluo Smart-Module manual]]'''« and »'''[[O2k-Fluo_Smart-Module#Fluorescence_dyes_and_settings|O2k-Fluo Smart-Module - Fluorescence dyes and settings]]'''« <br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
-- communicated by »[[Cardoso Luiza]]« and »[[Timon-Gomez Alba]]«
+|}
-|}
 ----
-*'''Nov- 21 Tu'''
+* '''May-21 Tu'''
 ----
-*'''Nov- 22 We'''
+* '''May-22 We''' - International Day for Biological Diversity
-----
-*'''Nov- 23 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"Thoughtful design and execution of O2k experiments result in data that become a powerhouse for addressing many mitochondrial respiration research questions. "''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Walsh MA, Musci RV, Jacobs RA, Hamilton KL (2023)  A practical perspective on how to develop, implement, execute, and reproduce high-resolution respirometry experiments: The physiologist's guide to an Oroboros O2k [[Walsh 2023 FASEB J|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[US CO Colorado Springs Jacobs RA]]« and »[[US CO Fort Collins Hamilton K]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
+----
+* '''May-23 Th'''
 ----
-*'''Nov- 24 Fr'''
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 48
+Week 22
-*'''Nov- 27 Mo'''
+* '''May-27 Mo'''
-----
-*'''Nov- 28 Tu'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Protocols that SUIT your research question'''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Using '''S'''ubstrate-'''U'''ncoupler-'''I'''nhibitor-'''T'''itration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay. <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocols]'''« and »'''[https://bioblast.at/index.php/SUITbrowser SUITbrowser]'''« <br>
+<br>
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Nov- 29 We'''
+* '''May-28 Tu'''
 ----
-*'''Nov- 30 Th'''
+* '''May-29 We'''
 ----
-*'''Dec- 01 Fr'''  <span style="color:red">World AIDS day</span>
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"We therefore hypothesized that the susceptibility to HIV-1 infection of FOXO1-inhibited resting T cells could be due to an increased cell metabolism. (...) Results obtained by high-resolution respirometry experiments of [human peripheral blood T cells] PBT (...) showed that [ROUTINE] respiration at the steady state was increased by [FOXO1 inhibitor] AS1842856."''' <br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-* Roux A, Leroy H, De Muylder B, Bracq L, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G, Mangeney M (2019) FOXO1 transcription factor plays a key role in T cell-HIV-1 interaction.  [[Roux 2019 PLoS Pathog|»Bioblast link«]]
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-:from the O2k-Network »[[FR Paris Bouillaud F]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
+----
+* '''May-31 Fr'''
 ----