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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 48
-*'''Nov- 27 Mo'''
+Week 21
-----
-*'''Nov- 28 Tu'''
+* '''May-20 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Protocols that SUIT your research question'''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-Using '''S'''ubstrate-'''U'''ncoupler-'''I'''nhibitor-'''T'''itration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay. <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocols]'''« and »'''[https://bioblast.at/index.php/SUITbrowser SUITbrowser]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
-----
-*'''Nov- 29 We'''
 ----
-*'''Nov- 30 Th'''
+* '''May-21 Tu'''
 ----
-*'''Dec- 01 Fr'''  <span style="color:red">World AIDS day</span>
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"We therefore hypothesized that the susceptibility to HIV-1 infection of FOXO1-inhibited resting T cells could be due to an increased cell metabolism. (...) Results obtained by high-resolution respirometry experiments of [human peripheral blood T cells] PBT (...) showed that [ROUTINE] respiration at the steady state was increased by [FOXO1 inhibitor] AS1842856."''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Roux A, Leroy H, De Muylder B, Bracq L, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G, Mangeney M (2019) FOXO1 transcription factor plays a key role in T cell-HIV-1 interaction.  [[Roux 2019 PLoS Pathog|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[FR Paris Bouillaud F]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
-:::: '''»[[AMI 2023 Jhansi IN]]«''' Dec 1-3
+----
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 49
+Week 22
-*'''Dec- 04 Mo'''
+* '''May-27 Mo'''
-----
-*'''Dec- 05 Tu'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Is DatLab 8 compatible with your operative system?'''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-The DatLab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
 <br>
-- »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
-- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Dec- 06 We'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
+----
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|link=https://wiki.oroboros.at/images/0/00/Cecatto_2023_MitoFit.pdf |MitoFit pdf]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"The capacity of FA oxidative phosphorylation (F-OXPHOS) with palmitoylcarnitine was up to 4 times higher than respiration with octanoylcarnitine. The optimal concentration of palmitoylcarnitine was 10 μM which corresponds to the total concentration of LC acylcarnitines in the brain."'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-* Cecatto C, Cardoso LHD, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Gnaiger E, Makrecka-Kuka M, Liepinsh E (2023) Fatty acid β-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. MitoFit Preprints 2023.10. https://doi.org/10.26124/mitofit:2023-0010
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-:from the O2k-Network »[[AT Innsbruck Oroboros]]« and »[[LV Riga Liepins E]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-*'''Dec- 07 Th'''
+* '''May-31 Fr'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"(…) L-Erg [L-Ergothioneine] treatment prevented and delayed cystine lithiasis in a cystinuria mouse model by increasing urinary cystine solubility and restored kidney GSH content and OXPHOS function."''' <br>
-* Mayayo-Vallverdú C, López de Heredia M, Prat E, González L, Espino Guarch M, Vilches C, Muñoz L, Asensi MA, Serra C, Llebaria A, Casado M, Artuch R, Garrabou G, Garcia-Roves PM, Pallardó FV, Nunes V (2023) The antioxidant l-Ergothioneine prevents cystine lithiasis in the Slc7a9-/- mouse model of cystinuria [[Mayayo-Vallverdu 2023 Redox Biol|»Bioblast link«]]
-:from the O2k-Network »[[ES Barcelona Moren C]]« and »[[ES Barcelona Garcia-Roves PM]]«
-|}
-*'''Dec- 08 Fr'''
 ----