Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 50
-*'''Dec- 11 Mo''' - International Mountain Day
+Week 21
+* '''May-20 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Mountain slopes can be difficult, but O<sub>2</sub> slopes can be smooth with DatLab'''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-<br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- »'''[https://wiki.oroboros.at/index.php/Flux_/_Slope#Smoothing.2C_noise.2C_and_sample_stability Smoothing in Flux/Slope]'''«<br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
+|}
-|}
 ----
-*'''Dec- 12 Tu'''
+* '''May-21 Tu'''
-:{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|MitoFit pdf]]
-| '''From preprint to Gold Open Access publication in J Biol Chem'''<br>
-* Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. [[Gnaiger 2023 J Biol Chem|»Bioblast link«]]
-|}
 ----
-*'''Dec- 13 We'''
+* '''May-22 We''' - International Day for Biological Diversity
-----
-*'''Dec- 14 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"HCR [High capacity runner rats] showed a consistent and robust trend of higher maximal oxygen consumption compared to LCR [low capacity runner rats], especially for long chain FAs [fatty acids]."''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Fleischman JY, Van den Bergh F, Collins NL, Bowers M, Beard DA, Burant CF (2023) Higher mitochondrial oxidative capacity is the primary molecular differentiator in muscle of rats with high and low intrinsic cardiorespiratory fitness. [[Fleischman 2023 Mol Metab|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[US MI Ann Arbor Beard DA]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-*'''Dec- 15 Fr'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 51
+Week 22
-*'''Dec- 18 Mo'''
-:::: '''»[[SBC 2023 Goa IN]]«''' Dec 18-20
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Why add azide after ascorbate and TMPD when measuring Complex IV activity? '''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-The O<sub><small>2</small></sub> flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O<sub><small>2</small></sub> flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement. <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[[Complex IV]]'''«, »'''[[Chemical background]] '''«  <br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Timon-Gomez Alba]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Dec- 19 Tu'''
+* '''May-28 Tu'''
 ----
-*'''Dec- 20 We'''
+* '''May-29 We'''
+----
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."''' <br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-* Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. [[Noone 2023 Cell Signal|»Bioblast link«]]
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-:from the O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-*'''Dec- 21 Th'''
+* '''May-31 Fr'''
-----
-*'''Dec- 22 Fr'''
 ----