Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 11
-* '''Mar-11 Mo'''
+Week 21
+* '''May-20 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O<sub>2</sub>] regime? '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-Long diffusion distances in the pfi result in an O<sub>2</sub> diffusion limitation, which is overcome by increasing the O<sub>2</sub> partial pressure inside the chamber. Proper instrumental background correction is a must and O<sub>2</sub> dependence of ROS production should be considered.   <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[[Permeabilized muscle fibers]]'''«, »'''[[Oxygen flux - instrumental background]]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
 ----
-* '''Mar-12 Tu'''
+* '''May-21 Tu'''
 ----
-* '''Mar-13 We'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
-| width="100" align="center" |[[Image:BEC-logo.png|100px|link=https://www.bioenergetics-communications.org/index.php/bec |Bioenergetics Communications]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''''Rewarded'' Open Access - compensating authors'''<br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-Together with the MiP''society'', BEC is raising funds to reward the first 10 papers accepted for publication in BEC in 2024: 600 EUR to attend EBEC2024 and 2-year MiP''membership''.
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
--»[https://whydonate.com/en/fundraising/BEC Support open access in science]«<br>
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
-- »[https://www.bioenergetics-communications.org/index.php/bec/about/submissions Submit a manuscript]«
 |}
 ----
-* '''Mar-14 Th'''
+* '''May-23 Th'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Mitochondrial ultrastructure and respiration are affected during EDL [''Extensor digitorum longus'' muscle] aging.''' <br>
-* Fernando R, Shindyapina AV, Ost M, Santesmasses D, Hu Y, Tyshkovskiy A, Yim SH, Weiss J, Gladyshev VN, Grune T, Castro JP (2023) Downregulation of mitochondrial metabolism is a driver for fast skeletal muscle loss during mouse aging.  [[Fernando 2023 Commun Biol|»Bioblast link«]]
-:from the O2k-Network »[[DE Leipzip Ost M]]«
-|}
 ----
-* '''Mar-15 Fr'''
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 12
+Week 22
-* '''Mar-18 Mo'''
+* '''May-27 Mo'''
-::::: '''[[EU-METAHEART MC2 Meeting 2024 Antalya TR]]''' Mar 18-21
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
+|}
 ----
-* '''Mar-19 Tu'''
+* '''May-28 Tu'''
-:::: 15:05 '''[[Gnaiger Erich|Erich Gnaiger]] presenting at [[EU-METAHEART MC2 Meeting 2024 Antalya TR]]''' - ''[[Donnelly_2024_EU-METAHEART_MC2_Meeting |Impact of hypoxia on mitochondrial function]]''
 ----
-* '''Mar-20 We'''
+* '''May-29 We'''
-:{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' What if ''Alert: Error in titrations!'' appears in your O2 analysis template file?  '''<br>
-The O2 analysis template recognizes discrepancies between the protocol selected in the first tab and the imported marks. Check the titration sequence and mark names against the protocol copied from the DLD file.<br>
-- »'''[[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|O<sub>2</sub> flux analysis]]'''«<br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
-|}
 ----
-* '''Mar-21 Th'''
+* '''May-30 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' How well do you handle your Hamilton syringes? What is a cleaning cycle?'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-"A syringe cleaning cycle consists of (1) filling an empty syringe with a washing solution while immersing [both the needle and] the base of the barrel into the washing solution, without fully removing the plunger, and (2) emptying the syringe into the waste beaker." <br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-- »'''[[MiPNet19.14 SOP Hamilton microsyringes]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Mar-22 Fr'''
+* '''May-31 Fr'''
 ----