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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 13
+Week 21
-* '''Mar-25 Mo'''
+* '''May-20 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Living cells or intact cells? '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.    <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[[Living cells]]'''«, »'''[[BEC 2020.1|Gnaiger 2020 BEC Mitochondrial physiology]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
 ----
-* '''Mar-26 Tu'''
+* '''May-21 Tu'''
 ----
-* '''Mar-27 We'''
+* '''May-22 We''' - International Day for Biological Diversity
-----
-* '''Mar-28 Th'''
 :{|
-| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-- »'''[[O2k-Feedback]]'''«<br>
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
+----
+* '''May-23 Th'''
 ----
-* '''Mar-29 Fr'''
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 14
+Week 22
-* '''Apr-01 Mo'''  April - Parkinson's Disease Awareness Month
+* '''May-27 Mo'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
+<br>
-|}
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
+|}
 ----
-* '''Apr-02 Tu'''
+* '''May-28 Tu'''
 ----
-* '''Apr-03 We'''
+* '''May-29 We'''
 ----
-* '''Apr-04 Th'''
+* '''May-30 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' "Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
-- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
 |}
 ----
-* '''Apr-05 Fr'''
+* '''May-31 Fr'''
 ----