Template:Current week ecosystem agenda: Difference between revisions
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| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] Β | | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] Β | ||
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br> | |'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br> | ||
:An 'O2 slope neg.' within Β± 2 pmolβs<sup>β1</sup>βmL<sup>β1</sup> is optimal (Β± 4 pmolβs<sup>β1</sup>βmL<sup>β1</sup> is acceptable).<br> | :An 'O2 slope neg.' within Β± 2 pmolβs<sup>β1</sup>βmL<sup>β1</sup> is optimal (Β± 4 pmolβs<sup>β1</sup>βmL<sup>β1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br> | ||
- Β»[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]Β« | - Β»[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]Β« | ||
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Revision as of 16:26, 1 July 2020
2020 Week 27
- Jun-29 Mo
Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?
- An 'O2 slope neg.' within Β± 2 pmolβsβ1βmLβ1 is optimal (Β± 4 pmolβsβ1βmLβ1 is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.
- Β» Air and zero calibrationΒ«
- An 'O2 slope neg.' within Β± 2 pmolβsβ1βmLβ1 is optimal (Β± 4 pmolβsβ1βmLβ1 is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.
- Jun-30 Tu
The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice
- from the O2k-Network Β»LV Riga Makrecka-Kuka MΒ«
- Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. Β»Bioblast linkΒ«
- Jul-01 We
Kick-off of the TRANSMIT crowdfunding campaign: NOW
- Bring the world of scientific research closer to the world of patients by supporting four cancer patientsβ associations.
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity.Information from: Gnaiger Erich et al β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Β»Bioblast linkΒ«
- Jul-03 Fr
Mitochondrial H2O2 flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration
- from the O2k-Network Β»US CO Fort Collins Chicco AJΒ«
- Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. Β»Bioblast linkΒ«
- from the O2k-Network Β»US CO Fort Collins Chicco AJΒ«