- high-resolution terminology - matching measurements at high-resolution
Zero calibration
Description
'Zero calibration is together with air calibration one of the two steps of the OroboPOS calibration. It is performed in the closed chamber after all the oxygen has been removed by the addition of dithionite, see MiPNet06.03 POS-Calibration-SOP. Unlike air calibration it is not necessary to perform a zero calibration each day.
Abbreviation: R0
Reference: D MiPNet12.08 O2k-Calibration
MitoPedia methods:
Respirometry
Zero oxygen calibration in the O2k
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
A zero calibration should be performed
- From time to time as a record of instrumental performance: use the template O2k-Calibration-list.
A zero calibration has to be performed
- When measurements are performed at low oxygen (<10 µM).
- Bracketing zero calibrations are required when measurements are performed near the limit of detection at oxygen concentrations close to 5 nM (0.005 µM).
- When a new or serviced OroboPOS is used for the first time.
- When a new membrane was applied to the sensor.
- When the gain is changed.
- When a new POS connector is used.
