MiPNet27.06 Prague BEC tutorial-Living Communications pmF: Difference between revisions
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<gallery mode=default perrow=9 widths="140px" heights="150px"> | <gallery mode=default perrow=9 widths="140px" heights="150px"> | ||
File: | File:Zuzana Korandova.jpg | '''[[Korandova Zuzana|Zuzana Korandová]]''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:Mracek Tomas.jpg |'''[[Mracek Tomas |Tomáš Mráček]]''', RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:Mracek Tomas.jpg |'''[[Mracek Tomas |Tomáš Mráček]]''', RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:PecinaP.JPG |'''[[Pecina Petr]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:PecinaP.JPG |'''[[Pecina Petr]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
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File:Profile-icon-9.png |11. '''Eliška Koňaříková''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:Profile-icon-9.png |11. '''Eliška Koňaříková''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:Houstek_J.jpg |12. '''[[Houstek Josef |Josef Houstek]]''', Prof, MD, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:Houstek_J.jpg |12. '''[[Houstek Josef |Josef Houstek]]''', Prof, MD, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File: | File:Zuzana Korandova.jpg |13. '''[[Korandova Zuzana|Zuzana Korandová]]''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File: | File:Barbora Kudnrovvska.jpg |14. '''[[Kudrnovska Barbora|Barbora Kudrnovská]]''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:Profile-icon-9.png |15. '''Aleksandra Marković''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:Profile-icon-9.png |15. '''Aleksandra Marković''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:Mracek Tomas.jpg |16. '''[[Mracek Tomas |Tomáš Mráček]]''', RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:Mracek Tomas.jpg |16. '''[[Mracek Tomas |Tomáš Mráček]]''', RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:PecinaP.JPG |17. '''[[Pecina Petr |Petr Pecina]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:PecinaP.JPG |17. '''[[Pecina Petr |Petr Pecina]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:PecinovaA.JPG |18. '''[[Pecinova Alena |Alena Pecinova]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:PecinovaA.JPG |18. '''[[Pecinova Alena |Alena Pecinova]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File: | File:Guillermo Puertas.jpg |19. '''[[Puertas Frias Guillermo|Guillermo Puertas Frias]]''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:SobotkaO.JPG|20. '''[[Sobotka Ondrej |Ondrej Sobotka]]''', MUDr, PhD, Faculty of Medicine, Charles University, Hradec Kralove, CZ | File:SobotkaO.JPG|20. '''[[Sobotka Ondrej |Ondrej Sobotka]]''', MUDr, PhD, Faculty of Medicine, Charles University, Hradec Kralove, CZ | ||
File:StankovaP.jpg|21. '''[[Stankova Pavla |Pavla Staňková]]''', Mgr, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ | File:StankovaP.jpg|21. '''[[Stankova Pavla |Pavla Staňková]]''', Mgr, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ | ||
File: | File:Maria Jose Saucedo.jpg |22. '''[[Saucedo Rodriges Maria Jose|Maria Jose Saucedo Rodriges]]''', Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:Katerina Tauchmannova.jpg |23. '''[[Tauchmannova Katerina |Kateřina Tauchmannová]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | File:Katerina Tauchmannova.jpg |23. '''[[Tauchmannova Katerina |Kateřina Tauchmannová]]''', PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ | ||
File:ZdrazilovaL.JPG|24. '''[[Zdrazilova Lucie |Lucie Zdrazilova]]''', MSc, First Faculty of Medicine,Charles University, Prague, CZ | File:ZdrazilovaL.JPG|24. '''[[Zdrazilova Lucie |Lucie Zdrazilova]]''', MSc, First Faculty of Medicine,Charles University, Prague, CZ |
Revision as of 11:28, 8 September 2022
Prague CZ, 2022 Sep 22. BEC tutorial-Living Communications: pmF — pre EMC2022 Prague CZ. |
MiPsociety (2022-09-22) Mitochondr Physiol Network
Abstract: BEC tutorial-Living Communications. Mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics. Preceding the EMC 2022 49th European Muscle Conference, Prague, Czech Republic.
The mitochondrial membrane potential is an element of the science of bioenergetics, linked to the control of respiratory flux and related mitochondrial functions. A PubMed search on ‘mitochondrial membrane potential’ yields nearly 40 000 results and 3442 for 2021 (search 2022-07-04), with a linear increase during the past 20 years. Chapter 8 on ‘Protonmotive pressure and respiratory control’ of Mitochondrial Pathways (Gnaiger 2020) introduces a novel perspective on Peter Mitchell’s protonmotive force, which incorporates the mitochondrial membrane potential. If you find the reading is tough, you are not alone. Join this BEC tutorial-Living Communications for an introduction into the relevant concepts of physical chemistry, which differ from misleading chapters in bioenergetics textbooks on potential gradients, Gibbs energy, protonmotive flow and force, and finally protonmotive pressure. This will introduce students (and teachers) to a new understanding of mitochondrial membrane potential and the protonmotive force, connecting the ideal gas equation, osmotic pressure, the Boltzmann constant and gas constant with Fick’s and Einstein’s diffusion equation. If theory gets tough, join for a follow-up retreat.
• O2k-Network Lab: AT_Innsbruck_Oroboros, CZ Prague Houstek J
Last update: 2022-08-14
This MiPevent is dedicated to Dr. Zdenek Drahota — one of the greatest mitochondrial physiologists of the Czech Republic — at his 90th birthday:
In collaboration with the Mitochondrial Physiology Society - see MiP2017
Venue and local organizers
- Laboratory of Bioenergetics
- Institute of Physiology CAS
- Videnska 1083
- 142 20 Prague 4
- Czech Republic
Zuzana Korandová, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
Tomáš Mráček, RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
Pecina Petr, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
Program
Thursday, Sep 22 Time Section Contents 10:00-11:30 Introduction Protonmotive force pmF = ΔmFH+
- Mitchell's theorems on four integrated coupling modules
- Is the protonmotive force — units [mV] or [kJ/mol] — a force?
- Is the "Gibbs energy" [kJ/mol] a force?
- Why did Peter Mitchell use the symbol Δp?
11:30-12:00 Coffee/tea Chat 12:00-13:00 Extension Four protonmotive theorems on protonmotive pressure ΔmΠH+
- Diffusion gradients
- Compartments: diffusion and osmotic pressure
- Hydrogen ions and counterions
- Matrix volume fraction and flux-pressure linearity
- Mitchell's theorems on four integrated coupling modules
pmF: a unifying theory of biology - from metabolism to physical chemistry
- Peter Mitchell's concept of the protonmotive force pmF is one of the grand unifying theories of biology, on par with Charles Darwin's theory of evolution, Gregor Mendel's rules of inheritance and classical genetics, and the structure of DNA resolved by Francis Crick, James Watson, and Rosalind Franklin. The pmF combines the disciplines of biochemistry (metabolism), cell biology (cellular ultrastructure), physiology (energy transformation), thermodynamics (chemical potential, Gibbs energy - better: Gibbs force), and physical chemistry (diffusion, electrochemistry).
- This BEC tutorial links different disciplines and describes different processes (transformations) by the same principles and relations of isomorphic quantities:
- metabolic reactions and translocation (scalar and vectorial)
- diffusion (from Fick's law to Einstein's diffusion equation)
- electrochemical potentials (compartmental differences versus gradients) and motive forces (of physics and thermodynamics - from the Boltzmann constant and gas constant to the electromotive constant)
- osmotic pressure (from the gas law to protonmotive pressure)
- Remember ZEN, zeNA — Section 8.2.8 - ∞2∞
- RM Pirsig (1974) Zen and the art of motorcycle maintenance. An inquiry into values. William Morrow & Company:418 pp.
- Thermodynamics (motorcycle maintenance) may be dull and tedious drudgery (without curiosity beyond “≡”) or a valuable and exciting art (if you seek for “=” ZEN). Transformation of dumb, dry and frigid equations into eloquent formulae radiating meaning and sparkling knowledge depends on motivation, skill and persistence (zeNA).
- » Compare numerical equivalence (symbol ≡) and physicochemical equality (symbol =).
Why?
- Why are mitochondria small? Why is LEAK respiration a non-linear (non-Ohmic) function of the mitochondrial membrane potential difference ΔΨp+?
- Why is the mitochondrial membrane potential difference ΔΨp+ — the chemical part of the pmF — not a force of physics? Similarly, the protonmotive force is not a force of physics. Why 'isomorphic' forces?
- Why can we start a chemical reaction (in a homogenous system) or compartmental diffusion (in a discontinuous system) at an infinitely large force - without the system exploding?
Consider some fundamental quantities
- Among the key isomorphic quantities are:
- advancement and stoichiometry as the determinants of transformation flows
- motive entity - this is what flows
- motive units for count, amount, and charge
- chemical and electric partial forces of the pmF
- Important distinctions:
- systems: closed, compartmental, open
- transformations: vectoral (along continuous gradients), vectorial (across discontinuous boundaries between compartments), scalar (within systems, without spatial direction)
- Gibbs energy (exergy), chemical potential, and metabolic force (Gibbs force)
- potential gradients versus potential differences
- protons p+ and hydrogen ions H+
- (chemiosmotic) pressure versus (protonmotive) force
- Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Fundamental relationships
- mt-Membrane potential and protonmotive force
- O2k-Potentiometry
- » O2k-Catalogue: O2k-TPP+ ISE-Module
- » O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
- » TPP - O2k-Procedures: Tetraphenylphosphonium
- » Specifications: MiPNet15.08 TPP electrode
- » Poster
- » Unspecific binding of TPP+
- » TPP+ inhibitory effect
- O2k-Potentiometry
- O2k-Fluorometry
- » O2k-Catalogue: O2k-FluoRespirometer
- » O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- » Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
- » TMRM - O2k-Procedures: TMRM
- O2k-Fluorometry
- O2k-Publications
Gibberish
- Forget all gibberish that you have learned — if not forgotten already — on textbook thermodynamics. If you are surprised by this suggestion, take a look at specific examples from
Force or pressure? - The linear flux-pressure law
- "For many decades the pressure-force confusion has blinded the most brilliant minds, reinforcing the expectation that Ohm’s linear flux-force law should apply to the hydrogen ion circuit and protonmotive force. .. Physicochemical principles explain the highly non-linear flux-force relation in the dependence of LEAK respiration on the pmF. The explanation is based on an extension of Fick’s law of diffusion and Einstein’s diffusion equation, representing protonmotive pressure ― isomorphic with mechanical pressure, hydrodynamic pressure, gas pressure, and osmotic pressure ― which collectively follow the generalized linear flux-pressure law."
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- » pressure = force × free activity
Lecturer and participants
Erich Gnaiger, PhD., Oroboros Instruments - author of
Participants
- Restricted number of participants: countmax = (29 + 1) x
1. Lukáš Alán, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
2. Zuzana Červinková, Prof, MD, PhD, Lab Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
3. Eva Doleželová, PhD, Institute of Parasitology, Ceske Budejovice, CZ
4. Zdenek Drahota, PhD, Lab of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
5. Olga Horakova, Laboratory of Adipose Tissue Biology, Institute of Physiology, Czech Academy of Sciences, CZ
7. Petra Janovska, PhD, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
8. Moustafa Elkalaf, MBBCh, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
9. René Endlicher, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
12. Josef Houstek, Prof, MD, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
13. Zuzana Korandová, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
14. Barbora Kudrnovská, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
16. Tomáš Mráček, RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
17. Petr Pecina, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
18. Alena Pecinova, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
19. Guillermo Puertas Frias, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
20. Ondrej Sobotka, MUDr, PhD, Faculty of Medicine, Charles University, Hradec Kralove, CZ
21. Pavla Staňková, Mgr, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
22. Maria Jose Saucedo Rodriges, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
23. Kateřina Tauchmannová, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
24. Lucie Zdrazilova, MSc, First Faculty of Medicine,Charles University, Prague, CZ
25. Petr Zouhar, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
26. František Galatík, Laboratory of Physiology, Faculty of Science, Charles University, Prague, CZ
27. Petr Kašík, Faculty of Science, Charles University, Prague, CZ
Registration and general information
- in preparation
- Informal, no registration fee - send Email to: [email protected]
- Limited number of participants
- Provide your name and affiliation (if you wish for the website)
- Provide a foto (if you wish for the website)
- in preparation
COVID-19
- The event will be held in accordance with current COVID regulations. A primary concern must be the safety of our participants and staff, which is why we reserve the right to cancel the event if there are any concerns/restrictions.
Recommended reading
- Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. https://doi.org/10.1016/j.bbabio.2011.09.018
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 - Chapter 8
- Gnaiger E (2021) The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. https://doi.org/10.26124/mitofit:200004.v2
BEC tutorials are listed as MitoGlobal Events.
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