O2k signals and output: Difference between revisions
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== Signal of the O2k-Core and add-on modules == | == Signal of the O2k-Core and add-on modules == | ||
[[Image:Oxygraph-2k-Concept.jpg|right|300px|O2k-Concept.jpg]] | [[Image:Oxygraph-2k-Concept.jpg|right|300px|O2k-Concept.jpg]] | ||
Three electronic channel types are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold (dual-data), for O2k-Chambers A and B (left and right), based on signals (numerical) sent at a fixed data sampling time interval (typical: 1 s or 2 s; range 0.2 s to >10 s): | Three electronic channel types are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold (dual-data), for O2k-Chambers A and B (left and right), based on signals (numerical) sent at a fixed data sampling time interval (typical: 1 s or 2 s; range 0.2 s to >10 s): | ||
Revision as of 17:01, 28 July 2015
Signal of the O2k-Core and add-on modules
Three electronic channel types are available in the O2k-MultiSensor system. All channels are available twofold (dual-data), for O2k-Chambers A and B (left and right), based on signals (numerical) sent at a fixed data sampling time interval (typical: 1 s or 2 s; range 0.2 s to >10 s):
- 1. O2 channel with electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The O2k-Core supports not only the O2 channel (O2 plug), but also the channel types 2 and 3 for the add-on modules.
- 2. Amp channel: The amperometric channel of the O2k (Amp plug), has electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp-Channel is used with the O2k-Fluo LED2-Module and the O2k-NO Amp-Module.
- 3. pX channel with electric potential (volt [V]) as the primary and raw signal (BNC pX plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref).
- 4. Events: Automatic events (e.g. from the TIP2k) and manually triggered events carry information on a time point during the experiment, a short event name (text), and a longer string of comments (text).
System channels, for instrumental control, not separated into O2k-Chamber A and B (single-data), based on signals (numerical) sent at the same fixed data sampling time interval:
- 5a. Block temperature: Measured block temperature equivalent to the experimental temperature in the O2k-Chambers at steady state [ยฐC].
- 5b. Peltier power (system channel): Relative heating or cooling power of the Peltier system.
- 5c. Barometric pressure (system channel): Measured barometric pressure [kPa].
- 5d. Ambient temperature (system channel): Measured temperature of room air (O2k-Series G upwards) [ยฐC].
- 5d. External temperature (system channel): Temperature measured with an independently placed thermistor (O2k-Series G upwards) [ยฐC].
NextGen-O2k: additional channel types are available in the NextGen-O2k:
- 6. Dual-fluo channel.
- 7. Spectrophotometric channel.
O2k output
The 'output' of a channel is the finally important infomation, which may be summarized in a data base. Three types of output can be derived from the signal of the O2k channels:
- I: Concentration. Examples: O2; Ca2+; amount of substrance per volume [1 ยตM = 1 ยตmolยทdm-3 = 1 nmolยทml-1].
- II: Flow, flux, rate. Examples: O2 consumption; H2O2 production; volume-specific flux is the negative or positive time derivative of concentration over time, frequently multiplied by 1,000 [pmolยทs-1ยทml-1]. Volume-specific flux normalized for the cell density [106ยทml-1] is flow [pmolยทs-1ยทml-1]. Volume-specific flux normalized for the mass density of the sample in the O2k-Chamber [mgยทml-1] is mass-specific flux [pmolยทs-1ยทmg-1]. Flux in any respiratory state normalized for flux in a defined reference state is a rate [dimensionless] or flux control ratio.
- III: Force, potential (e.g. TMRM and Safranin for mt-membrane potential; redox potential from cytochrome or NADH redox states).
- 1. O2 channel - O2k-Core: Output type I and II.
- 2a. Amp channel - O2k-Fluo LED2-Module: The focus of the output depends on the specific fluorophore. Output type I, II, or III, or any combination may relate to the primary level of interest.
- 2b. Amp channel - O2k-NO Amp-Module: Output type I and II.
- 3a. pX channel - O2k-TPP+ ISE-Module: Output type III.
- 3b. pX channel - O2k-pH ISE-Module: Output type I and II.
- 4. Events - O2k-Core and TIP2k
- 6. Single-fluo channel - NextGen-O2k: Plots of one selected wavelength can be selected for recording. Output type I(1), II(1), III(1) depends on the fluorophore.
- 7. Dual-fluo channel - NextGen-O2k: Plots of two selected wavelengths can be selected for simultaneous recording. Output type I(2), II(2), III(2) depends on the fluorophore.
- 8. Spectrophotometric channel - NextGen-O2k: Output type IV (three plots for redox states of cytochromes aa3, b, and c.