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== Bioenergetics Communications == | == Bioenergetics Communications == | ||
::::* Carlo Viscomi is Associate Editor of the journal '''[[Bioenergetics Communications]]''' | ::::* Carlo Viscomi is Associate Editor of the journal '''[[Bioenergetics Communications]]''' | ||
== NextGen-O2k == | |||
::::* Prof. Carlo Viscomi is Key-Opinion Leader (KOL) in the [[NextGen-O2k]] project. | |||
{{NextGen-O2k}} | |||
== Participated at == | == Participated at == |
Revision as of 08:49, 9 June 2020
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
Viscomi Carlo
MitoPedia topics: EAGLE
Name | Viscomi Carlo, PhD,
Associate Professor |
---|---|
Institution | Department of Biomedical Sciences,
University of Padova, IT |
Address | Via Ugo Bassi, 58/B, 35131 |
City | Padova |
State/Province | |
Country | Italy |
[email protected] | |
Weblink | |
O2k-Network Lab | IT Padova Viscomi C |
Bioenergetics Communications
- Carlo Viscomi is Associate Editor of the journal Bioenergetics Communications
NextGen-O2k
- Prof. Carlo Viscomi is Key-Opinion Leader (KOL) in the NextGen-O2k project.
MitoPedia: NextGen-O2k
- ยป NextGen-O2kยซ
Term | Abbreviation | Description |
---|---|---|
Hydrogen peroxide | H2O2 | Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS. |
Light-enhanced dark respiration | LEDR | Light-enhanced dark respiration LEDR is a sharp (negative) maximum of dark respiration in plants in response to illumination, measured immediately after switching off the light. LEDR is supported by respiratory substrates produced during photosynthesis and closely reflects light-enhanced photorespiration (Xue et al 1996). Based on this assumption, the total photosynthetic oxygen flux TP is calculated as the sum of the measured net photosynthetic oxygen flux NP plus the absolute value of LEDR. |
Mitochondrial membrane potential | mtMP, ฮฮจp+, ฮelFep+ [V] | The mitochondrial membrane potential difference, mtMP or ฮฮจp+ = ฮelFep+, is the electric part of the protonmotive force, ฮp = ฮmFeH+.
|
NADH-Module | The NADH-Module, is a component of the NextGen-O2k for simultaneous measurement of oxygen consumption and NAD(P)H autofluorescence. NAD(P)H autofluorescence is used to evaluate the redox state of the NAD(P)H-pool. The NADH-Module incorporates an UV light and NADH-Sensors which include a photodiode and specific filters. | |
NADH-Sensor | The NADH-Sensor has been developed as a part of the NADH-Module for simultaneous monitoring of oxygen consumption and NADH redox state. The NADH-Sensor is composed of a photodiode and equipped with three supergel R370 Italian blue filters (Rosco, US). | |
NextGen-O2k | ||
NextGen-O2k Technical developments | ||
Oxygen kinetics | Oxygen kinetics describes the dependence of respiration of isolated mitochondria or cells on oxygen partial pressure. Frequently, a strictly hyperbolic kinetics is observed, with two parameters, the oxygen pressure at half-maximum flux, p50, and maximum flux, Jmax. The p50 is in the range of 0.2 to 0.8 kPa for cytochrome c oxidase, isolated mitochondria and small cells, strongly dependent on Jmax and coupling state. | |
PB Light Source | The PhotoBiology Light Source (PBLS) has been designed as a part of the PB-Module to provide with an external source of light. This enables experiments for evaluating the production of O2 in the presence of light. The PBLS consists of one LED and one photodiode mounted on the PBLS head protected by a PMMA plastic cover. Three pairs of PBLS (white, blue, and red) are provided with the PB-Module. The light intensity can be regulated from 0 to 2750 ยตmolยทs-1ยทm-2 (red PBLS), from 0 to 3000 ยตmolยทs-1ยทm-2 (blue PBLS), from 0 to 3500 ยตmolยทs-1ยทm-2 (white PBLS). An integrated photodiode provides real-time measurement of the light intensity allowing for continuous adjustment to the desired value. | |
PB-Module | The PB-Module has been developed for conducting measurements of PhotoBiology, including photosynthesis. It consists of the PB Light Source and electronic components which are an integral part of the NextGen-O2k. Measurements are recorded and evaluated with the DatLab 8 software. | |
PhotoBiology | PB | PhotoBiology is the science of the effect of light on biological processes. This includes photosynthesis, photochemistry, photophysics, photomorphogenesis, vision, bioluminescence, circadian rhythms and photodynamic therapy. Phototoxicity results from non-ionizing radiation (i.e. ultraviolet, visible and infrared radiation). Non-ionizing radiation is any type of electromagnetic radiation that does not carry enough energy per quantum (photon energy below 10 eV) to completely remove an electron from an atom or molecule. When photons interact with molecules, the molecules can absorb the photon energy and become excited, reacting with surrounding molecules and stimulating "photochemical" and "photophysical" changes. Respiration may be affected by light during photosynthesis or in dark respiration, with the transient response of light-enhanced dark respiration. |
Photodecomposition | PD | Photodecomposition or photodegradation is the process of decay of organic material induced by increasing light intensity. Under aerobic conditions, the enhancement of photodecomposition by light intensity can be quantified by oxygen consumption in a controlled light regime. |
Photosynthesis | PS | Photosynthesis is the process that converts light energy into chemical energy which is subsequently transformed to the physiological energy demand. Photosynthesis has a light-dependent and light-independent (dark) phase. In plants, algae, and cynobacteria, light energy is absorbed during the light phase by the pigment chlorophyll and used to split water and generate adenosine triphosphate (ATP) and reducing power - nicotinamide adenine dinucleotide phosphate (NADPH), with the net production of O2 as a waste product. During the dark phase ATP and NADPH are used to synthesize carbohydrates from CO2 through the metabolic pathway called Calvin-Benson cycle. Oxygenic photosynthesis is responsible for producing and maintaining the oxygen concentration of the Earthโs atmosphere. In bacteria such as cyanobacteria, photosynthesis involves the plasma membrane and the cytoplasm. In eukaryotic cells (plants and algae), photosynthesis takes place in the chloroplasts. |
Q-Module | Q-Module | The Q-Module, developed for measuring the Q redox state and cyclic voltammetry, is supported by the NextGen-O2k and consists of the Q-Sensor, integrated electronic components in the O2k, and the DatLab software. |
Q-Sensor | The Q-Sensor has been designed as a part of the Q-Module for measurements with cyclic voltammetry and voltammetry, allowing for analysis of the Q redox state. The Q-Stopper with the reference electrode is called Q-Sensor, which is plugged in the NextGen-O2k. A three-electrode system is used to detect the Q redox state. Two of the three electrodes (glassy carbon and platinum electrode) are built into the Q-Stopper, while the reference electrode is removable (Reference-Electrode\2.4 mm). |
- Bioblast links: Q - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coenzyme Q
- ยป Coenzyme Q
- ยป Quinone, Ubiquinone Q; oxidized
- ยป Quinol, Ubiquinol QH2; reduced
- ยป Semiquinone
- ยป Coenzyme Q2
- ยป Q-redox state
- ยป Q-pools
- Coenzyme Q
- Mitochondrial pathways, respiratory Complexes, and Q
- ยป Q-cycle
- ยป Q-junction
- ยป Convergent electron flow
- ยป NS-pathway
- ยป FNS
- ยป FNSGp
- Mitochondrial pathways, respiratory Complexes, and Q
- ยป N-pathway
- ยป Reverse electron flow from CII to CI
- ยป CI
- ยป Rotenone
- ยป Amytal
- ยป Piericidin
- NextGen-O2k and Q-Module
- ยป NextGen-O2k
- ยป Q-Module
- ยป Q-Sensor
- ยป Cyclic voltammetry
- ยป Three-electrode system
- NextGen-O2k and Q-Module
- General
- Bioblast links: NADH - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- General
- ยป Extrinsic fluorophores
- ยป MiR05-Kit
- ยป ROS
- ยป Sensitivity
- General
- Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Phosphorylation pathway
- Phosphorylation pathway substrates
- ยป ADP
- ยป ATP
- ยป Inorganic phosphate
- Phosphorylation pathway substrates
- Phosphorylation pathway inhibitors
- ยป Oligomycin
- ยป Carboxyatractyloside
- ยป Atractyloside
- ยป Bongkrekik acid
- Phosphorylation pathway inhibitors
Coupling control
Respiratory complexes and coupling
- ยป Complex I
- ยป Complex III
- ยป Complex IV
- ยป Proton pump
- ยป Electron-transfer-pathway state
ATP production measurement
- Bioblast links: pH and protons - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- pH and protons
- ยป pH
- ยป hydrogen ion H+
- ยป hydron H+
- ยป hydronium ion H3O+
- ยป hydride H-
- ยป proton p+
- ยป pH buffering capacity
- ยป proton flux
- ยป proton pump versus hydrogen ion pump
- ยป proton leak
- ยป proton slip
- ยป protonmotive force
- pH and protons
- O2k-pH
- ยป O2k-Catalogue: O2k-pH ISE-Module
- ยป O2k-Manual pH electrode: MiPNet23.15 O2k-pH ISE-Module
- ยป O2k-SOP: MiPNet08.16 pH calibration
- ยป File:PH-Calibration-List.xls
- ยป NextGen-O2k, ratiometric: Carboxy SNARF 1
- ยป NextGen-O2k, ratiometric: HPTS
- ยป pH calibration buffers
- O2k-pH
- O2k-Publications
- HRFR - general
- ยป O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- ยป O2k signals and output
- ยป O2k-SOP: MiPNet14.06 Instrumental O2 background
- ยป MiPNet19.18A O2k-Series G: Start
- ยป ESD
- ยป O2k configuration
- ยป O2k control
- ยป O2k-FluoRespirometer
- ยป O2k-Main Unit#O2k-Series
- ยป Titration-Injection microPump
- ยป Compare: O2k-TPP+_ISE-Module
- HRFR - general
- DatLab
- Bioblast links: Calcium - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Bioblast links: PhotoBiology and plant physiology - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
PhotoBiology: photosynthesis
- ยป PhotoBiology
- ยป Photosynthesis
Plant physiology: respiration
NextGen-O2k and PB-Module
- ยป NextGen-O2k
- ยป PB-Module
- ยป PB-Sensor
Participated at
Labels:
Publications
Published | Reference | |
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Balmaceda 2024 Biochim Biophys Acta Mol Basis Dis | 2024 | Balmaceda V, Komlodi T, Szibor M, Gnaiger E, Moore AL, Fernandez-Vizarra E, Viscomi C (2024) The striking differences in the bioenergetics of brain and liver mitochondria are enhanced in mitochondrial disease. Biochim Biophys Acta Mol Basis Dis 1870:167033. https://doi.org/10.1016/j.bbadis.2024.167033 |
Yin 2021 Nat Commun | 2021 | Yin Z, Burger N, Kula-Alwar D, Aksentijeviฤ D, Bridges HR, Prag HA, Grba DN, Viscomi C, James AM, Mottahedin A, Krieg T, Murphy MP, Hirst J (2021) Structural basis for a complex I mutation that blocks pathological ROS production. Nat Commun 12:707. |
Peruzzotti-Jametti 2021 PLoS Biol | 2021 | Peruzzotti-Jametti L, Bernstock JD, Willis CM, Manferrari G, Rogall R, Fernandez-Vizarra E, Williamson JC, Braga A, van den Bosch A, Leonardi T, Krzak G, Kittel A, Beninca C, Vicario N, Tan S, Bastos C, Bicci I, Iraci N, Smith JA, Peacock B, Muller KH, Lehner PJ, Buzas EI, Faria N, Zeviani M, Frezza C, Brisson A, Matheson NJ, Viscomi C, Pluchino S (2021) Neural stem cells traffic functional mitochondria via extracellular vesicles. PLoS Biol 19:3001166. |
Szibor 2020 J Cell Mol Med | 2020 | Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet M, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. |
Peruzzotti-Jametti 2020 bioRxiv | 2020 | Peruzzotti-Jametti L, Bernstock JD, Manferrari G, Rogall R, Fernandez-Vizarra E, Williamson JC, Braga A, van den Bosch A, Leonardi T, Kittel A, Benincรก C, Vicario N, Tan S, Bastos C, Bicci I, Iraci N, Smith JA, Lehner PJ, Buzas EI, Faria N, Zeviani M, Frezza C, Brisson A, Matheson NJ, Viscomi C, Pluchino S (2020) Neural stem cells traffic functional mitochondria via extracellular vesicles to correct mitochondrial dysfunction in target cells. bioRxiv doi: https://doi.org/10.1101/2020.01.29.923441 . |
BEC 2020.1 doi10.26124bec2020-0001.v1 | 2020 | Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 |
Szibor 2019 Biochim Biophys Acta Bioenerg | 2019 | Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. |
Robb 2018 J Biol Chem | 2018 | Robb EL, Hall AR, Prime TA, Eaton S, Szibor M, Viscomi C, James AM, Murphy MP (2018) Control of mitochondrial superoxide production by reverse electron transport at complex I. J Biol Chem 293:9869-79. https://doi.org/10.1074/jbc.RA118.003647 |
Dogan 2018 Cell Metab | 2018 | Dogan SA, Cerutti R, Benincรก C, Brea-Calvo G, Jacobs HT, Zeviani M, Szibor M, Viscomi C (2018) Perturbed redox signaling exacerbates a mitochondrial myopathy. Cell Metab 28:764-77. |
Civiletto 2018 EMBO Mol Med | 2018 | Civiletto G, Dogan SA, Cerutti R, Fagiolari G, Moggio M, Lamperti C, Benincรก C, Viscomi C, Zeviani M (2018) Rapamycin rescues mitochondrial myopathy via coordinated activation of autophagy and lysosomal biogenesis. EMBO Mol Med 10:e8799. |
Profilo 2017 Biochim Biophys Acta | 2017 | Profilo E, Peรฑa-Altamira LE, Corricelli M, Castegna A, Danese A, Agrimi G, Petralla S, Giannuzzi G, Porcelli V, Sbano L, Viscomi C, Massenzio F, Palmieri EM, Giorgi C, Fiermonte G, Virgili M, Palmieri L, Zeviani M, Pinton P, Monti B, Palmieri F, Lasorsa FM (2017) Down-regulation of the mitochondrial aspartate-glutamate carrier isoform 1 AGC1 inhibits proliferation and N-acetylaspartate synthesis in Neuro2A cells. Biochim Biophys Acta 1863:1422-35. |
Pulliam 2014 Biochem J | 2014 | Pulliam DA, Deepa SS, Liu Y, Hill S, Lin AL, Bhattacharya A, Shi Y, Sloane L, Viscomi C, Zeviani M, Van Remmen H (2014) Complex IV-deficient Surf1(-/-) mice initiate mitochondrial stress responses. Biochem J 462:359-71. |
Abstracts
Published | Reference | |
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Kovarova 2015 Abstract MiP2015 | 2015 | Tissue- and species-specific differences in cytochrome c oxidase assembly induced by SURF1 defects. |
Fernandez-Vizarra 2013 Abstract IOC80 | 2013 | Fernandez-Vizarra E (2013) Testing OXPHOS biogenesis and function in mitochondrial disease models. Mitochondr Physiol Network 18.09. |