Bakkman 2007 ActaPhysiol: Difference between revisions
No edit summary |
No edit summary |
||
Line 16: | Line 16: | ||
}} | }} | ||
__TOC__ | __TOC__ | ||
== MitoEAGLE ''V''<sub>O<sub>2</sub>max</sub>/BME | == MitoEAGLE ''V''<sub>O<sub>2</sub>max</sub>/BME database == | ||
:::* Human vastus lateralis | :::* Human vastus lateralis | ||
Line 30: | Line 30: | ||
:::* ''J''<sub>O<sub>2</sub>,''P''</sub>(NS) = 97.7 µmol·s<sup>-1</sup>·kg<sup>-1</sup> wet muscle mass (37 °C) | :::* ''J''<sub>O<sub>2</sub>,''P''</sub>(NS) = 97.7 µmol·s<sup>-1</sup>·kg<sup>-1</sup> wet muscle mass (37 °C) | ||
::::* ''J''<sub>O<sub>2</sub>,''P''</sub>(PM) = 62.5 µmol·s<sup>-1</sup>·kg<sup>-1</sup> wet muscle mass (37 °C) | ::::* ''J''<sub>O<sub>2</sub>,''P''</sub>(PM) = 62.5 µmol·s<sup>-1</sup>·kg<sup>-1</sup> wet muscle mass (37 °C) | ||
::::* ''J''<sub>O<sub>2</sub>,''P''</sub>(NS) = ''J''<sub> | ::::* ''J''<sub>O<sub>2</sub>,''P''</sub>(NS) = ''J''<sub>O<sub>2</sub>,''P''</sub>(PM)/0.64 | ||
::::* 8.3 µM mt-protein/mg ''m''<sub>w</sub> | ::::* 8.3 µM mt-protein/mg ''m''<sub>w</sub> | ||
---- | ---- |
Revision as of 05:29, 8 February 2020
Bakkman L, Sahlin K, Holmberg HC, Tonkonogi M (2007) Quantitative and qualitative adaptation of human skeletal muscle mitochondria to hypoxic compared with normoxic training at the same relative work rate. Acta Physiol (Oxford) 190:243–51. |
Bakkman L, Sahlin K, Holmberg HC, Tonkonogi M (2007) Acta Physiol (Oxford)
Abstract: Aim: To investigate if training during hypoxia (H) improves the adaptation of muscle oxidative function compared with normoxic (N) training performed at the same relative intensity.
Method: Eight untrained volunteers performed one-legged cycle training during 4 weeks in a low-pressure chamber. One leg was trained under N conditions and the other leg under hypobaric hypoxia (526 mmHg) at the same relative intensity as during N (65% of maximal power output, Wmax). Muscle biopsies were taken from vastus lateralis before and after the training period. Muscle samples were analysed for the activities of oxidative enzymes [citrate synthase (CS) and cytochrome c oxidase (COX)] and mitochondrial respiratory function.
Results: Wmax increased with more than 30% over the training period during both N and H. CS activity increased significantly after training during N conditions (+20.8%, P < 0.05) but remained unchanged after H training (+4.5%, ns) with a significant difference between conditions (P < 0.05 H vs. N). COX activity was not significantly changed by training and was not different between exercise conditions [+14.6 (N) vs. -2.3% (H), ns]. Maximal ADP stimulated respiration (state 3) expressed per weight of muscle tended to increase after N (+31.2%, P < 0.08) but not after H training (+3.2%, ns). No changes were found in state four respiration, respiratory control index, P/O ratio, mitochondrial Ca2+ resistance and apparent Km for oxygen.
Conclusion: The training-induced increase in muscle oxidative function observed during N was abolished during H. Altitude training may thus be disadvantageous for adaptation of muscle oxidative function. • Keywords: altitude, apparent Km for oxygen, citrate synthase, cytochrome c oxidase, hypoxic exercise, mitochondrial function, oxidative capacity, respiration, latent mitochondrial dysfunction • Bioblast editor: Gnaiger E
MitoEAGLE VO2max/BME database
- Human vastus lateralis
- 3 females & 5 males
- 27.4 years
- Untrained
- H = 1.75 m
- M = 74.9 kg
- BME = 0.19
- BMI = 24.5 kg·m-2
- VO2max/M = 44.5 mL·min-1·kg-1
- Isolated mitochondria; 25 °C; PMP; conversions: Gnaiger 2009 Int J Biochem Cell Biol
- JO2,P(NS) = 97.7 µmol·s-1·kg-1 wet muscle mass (37 °C)
- JO2,P(PM) = 62.5 µmol·s-1·kg-1 wet muscle mass (37 °C)
- JO2,P(NS) = JO2,P(PM)/0.64
- 8.3 µM mt-protein/mg mw
References: BME and VO2max
- » VO2max
Reference | |
---|---|
Bakkman 2007 ActaPhysiol | Bakkman L, Sahlin K, Holmberg HC, Tonkonogi M (2007) Quantitative and qualitative adaptation of human skeletal muscle mitochondria to hypoxic compared with normoxic training at the same relative work rate. Acta Physiol (Oxford) 190:243–51. |
Boushel 2007 Diabetologia | Boushel RC, Gnaiger E, Schjerling P, Skovbro M, Kraunsoee R, Dela F (2007) Patients with Type 2 diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50:790-6. |
Chambers 2020 J Appl Physiol (1985) | Chambers TL, Burnett TR, Raue U, Lee GA, Finch WH, Graham BM, Trappe TA, Trappe S (2020) Skeletal muscle size, function, and adiposity with lifelong aerobic exercise. J Appl Physiol (1985) 128:368–78. |
Daussin 2008 Am J Physiol Regul Integr Comp Physiol | Daussin FN, Zoll J, Dufour SP, Ponsot E, Lonsdorfer-Wolf E, Doutreleau S, Mettauer B, Piquard F, Geny B, Richard R (2008) Effect of interval versus continuous training on cardiorespiratory and mitochondrial functions: relationship to aerobic performance improvements in sedentary subjects. Am J Physiol Regul Integr Comp Physiol 295:R264-72. |
Garnier 2005 FASEB J | Garnier A, Fortin D, Zoll J, N'Guessan B, Mettauer B, Lampert E, Veksler V, Ventura-Clapier R (2005) Coordinated changes in mitochondrial function and biogenesis in healthy and diseased human skeletal muscle. FASEB J 19:43-52. |
Gnaiger 2015 Scand J Med Sci Sports | Gnaiger E, Boushel R, Søndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612 |
Gnaiger 2019 MiP2019 | OXPHOS capacity in human muscle tissue and body mass excess – the MitoEAGLE mission towards an integrative database (Version 6; 2020-01-12). |
Loe 2013 PLOS ONE | Loe H, Rognmo Ø, Saltin B, Wisløff U (2013) Aerobic capacity reference data in 3816 healthy men and women 20-90 years. PLOS ONE 8:e64319. |
Mettauer 2001 J Am Coll Cardiol | Mettauer B, Zoll J, Sanchez H, Lampert E, Ribera F, Veksler V, Bigard X, Mateo P, Epailly E, Lonsdorfer J, Ventura-Clapier R (2001) Oxidative capacity of skeletal muscle in heart failure patients versus sedentary or active control subjects. J Am Coll Cardiol 38:947-54. |
Mogensen 2006 J Physiol | Mogensen M, Bagger M, Pedersen PK, Fernström M, Sahlin K (2006) Cycling efficiency in humans is related to low UCP3 content and to type I fibres but not to mitochondrial efficiency. J Physiol 571:669-81. |
N'Guessan 2004 Mol Cell Biochem | N'Guessan B, Zoll J, Ribera F, Ponsot E, Lampert E, Ventura-Clapier R, Veksler V, Mettauer B (2004) Evaluation of quantitative and qualitative aspects of mitochondrial function in human skeletal and cardiac muscles. Mol Cell Biochem 256-257:267-80. |
Pesta 2011 Am J Physiol Regul Integr Comp Physiol | Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301:R1078–87. |
Ponsot 2006 J Appl Physiol (1985) | Ponsot E, Dufour SP, Zoll J, Doutrelau S, N'Guessan B, Geny B, Hoppeler H, Lampert E, Mettauer B, Ventura-Clapier R, Richard R (2006) Exercise training in normobaric hypoxia in endurance runners. II. Improvement of mitochondrial properties in skeletal muscle. J Appl Physiol (1985) 100:1249-57. |
Pribis 2010 Nutrients | Pribis P, Burtnack CA, McKenzie SO, Thayer J (2010) Trends in body fat, body mass index and physical fitness among male and female college students. Nutrients 2:1075-85. |
Raboel 2009 Diabetes Obes Metab | Raboel R, Hojberg PM, Almdal T, Boushel RC, Haugaard SB, Madsbad S, Dela F (2009) Improved glycaemic control decreases inner mitochondrial membrane leak in type 2 diabetes. Diabetes Obes Metab 11:355-60. |
Rasmussen 2001 Am J Physiol Endocrinol Metab | Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280:E301-7. |
Rasmussen 2003 Eur J Physiol | Rasmussen UF, Krustrup P, Kjaer M, Rasmussen HN (2003) Human skeletal muscle mitochondrial metabolism in youth and senescence: no signs of functional changes in ATP formation and mitochondrial oxidative capacity. Pflugers Arch – Eur J Physiol 446:270-78. |
Zoll 2002 J Physiol | Zoll J, Sanchez H, N'Guessan B, Ribera F, Lampert E, Bigard X, Surrurier B, Fortin D, Geny B, Veksler V, Ventura-Clapier R, Mettauer B (2002) Physical activity changes the regulation of mitochondrial respiration in human skeletal muscle. J Physiol 543:191-200. |
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Stress:Oxidative stress;RONS Organism: Human Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria
Regulation: Oxygen kinetics Coupling state: OXPHOS Pathway: N
latent mitochondrial dysfunction, MitoEAGLE BME, BMI, VO2max, BME