Description
Coupling control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial substrate state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ETS). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, Jref, is defined by taking Jref as the maximum flux, i.e. flux in the ETS state, E, such that the lower and upper limits of CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for intact cells, L/E and R/E.
Abbreviation: CCR
Reference: Flux control ratio
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
- Free OXPHOS capacity, βP = P-L
- OXPHOS coupling efficiency, jβP = βP/P = 1-L/P
- Free ROUTINE activity, βR = R-L
- ROUTINE coupling efficiency, jβR = βR/R = 1-L/R
- LEAK control ratio, L/E
- Free ETS capacity, βE = E-L
- ETS coupling efficiency, jβE = βE/E = 1-L/E
- netOXPHOS control ratio, βP/E=(P-L)/E
- Free OXPHOS capacity, βP = P-L
- netROUTINE control ratio, βR/E=(R-L)/E
- Free ROUTINE activity, βR = R-L
- More details: Β»ETS coupling efficiency
- OXPHOS control ratio, P/E
- Excess E-P capacity, ExP = E-P
- Excess E-P capacity factor, jExP = ExP/E = 1-P/E
- Excess E-R capacity, ExR = E-R
- Excess E-R capacity factor, jExR = ExR/E = 1-R/E
Compare
- Β» Coupling control factor, CCF = 1-CCR
- Β» Respiratory acceptor control ratio, RCR