Difference between revisions of "Coupling-control state"
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|description='''Coupling control states''' are defined in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as [[LEAK]], [[OXPHOS]], and [[ETS]] states of respiration (''L, P, E'') in any [[substrate control state]] which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the [[phosphorylation system]], titration of ADP and uncouplers. In intact cells, the coupling control states are [[LEAK]], [[ROUTINE]], and [[ETS]] states of respiration (''L, R, E''). [[Coupling control protocol]]s induce these coupling control states sequentially at a constant [[substrate control state]]. | |description='''Coupling control states''' are defined in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as [[LEAK]], [[OXPHOS]], and [[ETS]] states of respiration (''L, P, E'') in any [[substrate control state]] which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the [[phosphorylation system]], titration of ADP and uncouplers. In intact cells, the coupling control states are [[LEAK]], [[ROUTINE]], and [[ETS]] states of respiration (''L, R, E''). [[Coupling control protocol]]s induce these coupling control states sequentially at a constant [[substrate control state]]. | ||
|info=[[Gnaiger 2009 Int J Biochem Cell Biol]] | |info=[[Gnaiger 2009 Int J Biochem Cell Biol]] | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
Revision as of 23:15, 27 January 2016
- high-resolution terminology - matching measurements at high-resolution
Coupling-control state
Description
Coupling control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK, OXPHOS, and ETS states of respiration (L, P, E) in any substrate control state which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the phosphorylation system, titration of ADP and uncouplers. In intact cells, the coupling control states are LEAK, ROUTINE, and ETS states of respiration (L, R, E). Coupling control protocols induce these coupling control states sequentially at a constant substrate control state.
Reference: Gnaiger 2009 Int J Biochem Cell Biol
MitoPedia methods: Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Coupling states: 
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Last edit: Gnaiger E, 2015-10-04
