Difference between revisions of "Coupling-control state"

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Coupling-control state

Description

Coupling control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK, OXPHOS, and ETS states of respiration (L, P, E) in any substrate control state which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the phosphorylation system, titration of ADP and uncouplers. In intact cells, the coupling control states are LEAK, ROUTINE, and ETS states of respiration (L, R, E). Coupling control protocols induce these coupling control states sequentially at a constant substrate control state.

Abbreviation: CCS

Reference: Gnaiger 2009 Int J Biochem Cell Biol

MitoPedia concepts: Respiratory state, SUIT state

MitoPedia methods: Respirometry

The Blue Book 2014: Fig. 2.4.

Coupling states: -

Last edit: Gnaiger E, 2015-10-04

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 {{MitoPedia
+|abbr=''CCS''
 |description='''Coupling control states''' are defined in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as [[LEAK]], [[OXPHOS]], and [[ETS]] states of respiration (''L, P, E'') in any [[substrate control state]] which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the [[phosphorylation system]], titration of ADP and uncouplers. In intact cells, the coupling control states are [[LEAK]], [[ROUTINE]], and [[ETS]] states of respiration (''L, R, E''). [[Coupling control protocol]]s induce these coupling control states sequentially at a constant [[substrate control state]].
 |info=[[Gnaiger 2009 Int J Biochem Cell Biol]]