Difference between revisions of "Garedew 2005 Eur Shock Soc"
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|abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k]], combining instrumental design, electronics and task-specific DatLab software. | |abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k]], combining instrumental design, electronics and task-specific DatLab software. | ||
Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP-2k]] is applied for kinetics as a modular component of the Oxygraph-2k. [[DatLab]] 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP-2k]] is applied for kinetics as a modular component of the Oxygraph-2k. [[DatLab]] 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | ||
|mipnetlab=AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen Duerr P | |mipnetlab=AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen Duerr P | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
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|kinetics=Inhibitor; Uncoupler | |kinetics=Inhibitor; Uncoupler | ||
|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential | |topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential | ||
|additional=Instrumental and methodological aspects | |||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Revision as of 11:55, 11 November 2011
| Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS Oxygraph-2k. Proc. 11th Congress Eur. Shock Soc., Vienna, Austria (Redl H, ed) Medimond, Bologna: 107-111. |
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Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen Duerr P, Gnaiger E (2005)
Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the OROBOROS Oxygraph-2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP-2k is applied for kinetics as a modular component of the Oxygraph-2k. DatLab 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
• O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen Duerr P
Labels:
Organism: Human
Tissue;cell: Fibroblast
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k, TIP2k
Instrumental and methodological aspects
