Difference between revisions of "Gnaiger 2008 POS"
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|preparations=Intact Cell; Cultured; Primary | |preparations=Intact Cell; Cultured; Primary | ||
|kinetics=Oxygen | |kinetics=Oxygen | ||
|additional=Instrumental and methodological aspects | |||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
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===Highlights=== | ===Highlights=== | ||
Several features distinguish '''[[high-resolution respirometry]]''' from traditional oxygraphs, combined in the new [[Oxygraph-2k]] (O2k, OROBOROS INSTRUMENTS, Innsbruck, Austria; Figure 3). The specifications are unique: the limit of detection of respiratory flux is 1 pmol∙s-1∙cm-3 (0.001 µM∙s-1) and the limit of detection of oxygen concentration extends to 0.005 µM O2. For the non-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of the system is exhausted slowly, allowing sufficient time to evaluate the stability of respiratory activity in each metabolic state and to permit complex titration regimes in intact cells or in permeabilized cells and tissues. To increase throughput in research with cell cultures and in the pharmacological arena, user-friendly features make it possible to apply several instruments in parallel, each O2k with two independent chambers. | Several features distinguish '''[[high-resolution respirometry]]''' from traditional oxygraphs, combined in the new [[Oxygraph-2k]] (O2k, OROBOROS INSTRUMENTS, Innsbruck, Austria; Figure 3). The specifications are unique: the limit of detection of respiratory flux is 1 pmol∙s-1∙cm-3 (0.001 µM∙s-1) and the limit of detection of oxygen concentration extends to 0.005 µM O2. For the non-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of the system is exhausted slowly, allowing sufficient time to evaluate the stability of respiratory activity in each metabolic state and to permit complex titration regimes in intact cells or in permeabilized cells and tissues. To increase throughput in research with cell cultures and in the pharmacological arena, user-friendly features make it possible to apply several instruments in parallel, each O2k with two independent chambers. | ||
Revision as of 11:49, 11 November 2011
| Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. |
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Gnaiger E (2008) John Wiley
Abstract: Several features distinguish high-resolution respirometry from traditional oxygraphs, combined in the new Oxygraph-2k.
• O2k-Network Lab: AT_Innsbruck_Gnaiger E
Labels:
Organism: Mouse
Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k, TIP2k
Instrumental and methodological aspects
Highlights
Several features distinguish high-resolution respirometry from traditional oxygraphs, combined in the new Oxygraph-2k (O2k, OROBOROS INSTRUMENTS, Innsbruck, Austria; Figure 3). The specifications are unique: the limit of detection of respiratory flux is 1 pmol∙s-1∙cm-3 (0.001 µM∙s-1) and the limit of detection of oxygen concentration extends to 0.005 µM O2. For the non-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of the system is exhausted slowly, allowing sufficient time to evaluate the stability of respiratory activity in each metabolic state and to permit complex titration regimes in intact cells or in permeabilized cells and tissues. To increase throughput in research with cell cultures and in the pharmacological arena, user-friendly features make it possible to apply several instruments in parallel, each O2k with two independent chambers.
