MiPNet17.04 CitrateSynthase: Difference between revisions

Latest revision as of 15:56, 1 February 2021

MiPNet17.04 CitrateSynthase

[[Has title:: Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.]]

» [[Has info:: »Versions]]

Was written by::Oroboros (Was published in year::2020-04-18) Was published in journal::Mitochondr Physiol Network

Abstract: [[has abstract::Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.

Version 04: New Section 6. Normalization of respiratory flux for CS activity]] • Keywords: has publicationkeywords::Normalization of rate

• O2k-Network Lab: Was published by MiPNetLab::AT Innsbruck Oroboros

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

Author contributions

This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project MitoCom Tyrol, funded by the Tyrolian Government and the European Regional Development Fund (ERDF).

Last updates: contribution to H2020 Project NextGen-O2k.

Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Rate

» Normalization of rate

» Flow

» Oxygen flow

» Flux

» Oxygen flux

» Flux control ratio

» Coupling-control ratio

» Pathway control ratio

» Flux control efficiency

Quantities for normalization

» Count in contrast to Number

» Mitochondrial marker

» O2k-Protocols: mitochondrial and marker-enzymes

» Citrate synthase activity

General

» Extensive quantity

» Specific quantity

» Advancement

» Motive unit

» Iconic symbols

Related keyword lists

» Keywords: Concentration and pressure

Labels: MiParea: MiP area::Respiration, MiP area::Instruments;methods

Preparation: Preparation::Permeabilized cells, Preparation::Permeabilized tissue, Preparation::Homogenate, Preparation::Isolated mitochondria, Preparation::Enzyme Enzyme: Enzyme::Marker enzyme

HRR: Instrument and method::Oxygraph-2k, Instrument and method::O2k-Protocol

additional label::Mt- and marker-enzymes, additional label::Normalization of rate

@@ Line 1: / Line 1: @@
+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme.
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.
 |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/4/40/MiPNet17.04_CitrateSynthase.pdf|Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet17.04_CitrateSynthase.pdf Versions]
 |authors=Oroboros
 |year=2020-04-18
 |journal=Mitochondr Physiol Network
-|abstract=Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger E (2020) Laboratory protocol: Citrate synthase. Mitochondrial marker  enzyme. Mitochondr Physiol Network 17.04(04):1-11.
+|abstract=Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: [[Citrate synthase]] a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.
-{{MiPNet pdf page linking to MitoPedia}}
-:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
+Version 04: New Section '''6. Normalization of respiratory flux for CS activity'''
+|keywords=Normalization of rate
 |mipnetlab=AT Innsbruck Oroboros
 }}
+{{MiPNet pdf page linking to MitoPedia}}
+== Author contributions ==
+:::: This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project ''MitoCom Tyrol'', funded by the Tyrolian Government and the European Regional Development Fund (ERDF).
+:::: Last updates: contribution to H2020 Project NextGen-O2k.
+{{Template:Keywords: Normalization}}
 {{Labeling
-|area=Instruments;methods
+|area=Respiration, Instruments;methods
 |preparations=Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme
 |enzymes=Marker enzyme
-|instruments=O2k-Protocol
+|instruments=Oxygraph-2k, O2k-Protocol
-|additional=Mt- and marker-enzymes
+|additional=Mt- and marker-enzymes, Normalization of rate
 }}
-{{OROBOROS header page name}}