MiPNet17.04 CitrateSynthase: Difference between revisions

Latest revision as of 15:56, 1 February 2021

MiPNet17.04 CitrateSynthase

Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.

» »Versions

Oroboros (2020-04-18) Mitochondr Physiol Network

Abstract: Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.

Version 04: New Section 6. Normalization of respiratory flux for CS activity • Keywords: Normalization of rate

• O2k-Network Lab: AT Innsbruck Oroboros

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

Author contributions

This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project MitoCom Tyrol, funded by the Tyrolian Government and the European Regional Development Fund (ERDF).

Last updates: contribution to H2020 Project NextGen-O2k.

Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Rate

» Normalization of rate

» Flow

» Oxygen flow

» Flux

» Oxygen flux

» Flux control ratio

» Coupling-control ratio

» Pathway control ratio

» Flux control efficiency

Quantities for normalization

» Count in contrast to Number

» Mitochondrial marker

» O2k-Protocols: mitochondrial and marker-enzymes

» Citrate synthase activity

General

» Extensive quantity

» Specific quantity

» Advancement

» Motive unit

» Iconic symbols

Related keyword lists

» Keywords: Concentration and pressure

Labels: MiParea: Respiration, Instruments;methods

Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme Enzyme: Marker enzyme

HRR: Oxygraph-2k, O2k-Protocol

Mt- and marker-enzymes, Normalization of rate

@@ Line 1: / Line 1: @@
+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://www.oroboros.at/?O2k-Protocols|O2k-Protocols contents]]Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger E (2012) Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 17.04.
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.
-|info=[[Media:MiPNet17.04 CitrateSynthase.pdf|'''Open Access-MiPNet17.04.pdf''']] ; [http://www.bioblast.at/index.php/File:MiPNet17.04_CitrateSynthase.pdf Versions]
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/4/40/MiPNet17.04_CitrateSynthase.pdf|Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet17.04_CitrateSynthase.pdf Versions]
-|authors=OROBOROS
+|authors=Oroboros
-|year=2012
+|year=2020-04-18
 |journal=Mitochondr Physiol Network
-|abstract=Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al 1970; Willimas et al 1986; Hood et al 1989), although this role of CS has been questioned in developmental (Drahota et al 2004), age-related studies (Marin-Garcia et al 1998), physical training ([[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta et al 2011]]) and cardiac disease studies ([[Lemieux 2011 Int J Biochem Cell Biol|Lemieux et al 2011]]). Proliferation of mitochondria in pathological states is sometimes associated with an increase in CS activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein or per mt-respiratory capacity ([[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]). Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications ([[Kuznetsov 2002 Analyt Biochem|Kuznetsov et al 2002]]; [[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]; [[Huetter 2004 Biochem J|Hütter el al 2004]]).
+|abstract=Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: [[Citrate synthase]] a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.
-:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
-:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
+Version 04: New Section '''6. Normalization of respiratory flux for CS activity'''
-|mipnetlab=AT_Innsbruck_OROBOROS
+|keywords=Normalization of rate
-|discipline=Mitochondrial Physiology
+|mipnetlab=AT Innsbruck Oroboros
-|articletype=Protocol; Manual, MiPNet-online Publication
 }}
+{{MiPNet pdf page linking to MitoPedia}}
+== Author contributions ==
+:::: This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project ''MitoCom Tyrol'', funded by the Tyrolian Government and the European Regional Development Fund (ERDF).
+:::: Last updates: contribution to H2020 Project NextGen-O2k.
+{{Template:Keywords: Normalization}}
 {{Labeling
-|area=Instruments;methods, mt-Biogenesis;mt-density
+|area=Respiration, Instruments;methods
-|preparations=Enzyme
+|preparations=Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme
-|enzymes=Marker Enzyme
+|enzymes=Marker enzyme
-|instruments=Protocol
+|instruments=Oxygraph-2k, O2k-Protocol
-|discipline=Mitochondrial Physiology
+|additional=Mt- and marker-enzymes, Normalization of rate
-|articletype=Protocol; Manual, MiPNet-online Publication
 }}
-== Further information ==
-:>> Continue the discussion:[[Talk:MiPNet17.04 CitrateSynthase]]