MiPNet17.14 IOC72 Schroecken AT
O2k-Workshop on High-Resolution Respirometry: O2k-Basic
Schroecken, Vorarlberg, Austria 2012 December 05 to 10
This workshop is a component of the K-Regio project MitoCom Tyrol.
Contact: [email protected] [email protected]
1. Workshop materials
For those who have one (or more) O2k-instruments in the lab, I recommend to bring along the O2k-Compendium (O2k-Manual). During the Workshop, we will repeatedly refer to the O2k-Manual (Open access).
The fully updated O2k-Manual, O2k-Protocols, and O2k-References (pdf reprints) will be available on your personal OROBOROS-USB included in the workshop material. Bring your laptop or use our Notebooks during the workshop, making yourself familiar with the O2k-FileFinder while our tutors can provide immediate guidance. You can download the DatLab software and many DatLab demo files from the OROBOROS-USB, such that you can practice the use of DatLab. Although our software is known as user-friendly, DatLab is always a key part of a hands-on workshop.
A comprehensive methodological overview on high-resolution respirometry (Pesta and Gnaiger 2012), is recommended for reading as a good preparation for the workshop. In addition to the literature, a random or targeted walk through the O2k-Catalogue provides lots of information on the various parts of the O2k and its modular extensions.
2. Final programme
The programme can be accessed and downloaded here: soon
We keep the programme flexible, and all theoretical parts are discussion-oriented and interactive. In addition to the practical sessions, we will cover some fundamentals of mitochondrial physiology and protocol design. If you want to refresh your memory on a few conventional bioenergetic terms, take a look at the Bioblast Glossary on respiratory states - we will take some time to discuss the rationale for an extension of bioenergetics to mitochondrial physiology.
>>> Summary of links
- O2k-Manual: http://www.oroboros.at/?O2k-Manual
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
- Further reading: http://www.oroboros.at/?IOC-Reading
- O2k-Catalogue_OROBOROS
- IOC72 Programme: available soon
- Glossary:_Respiratory_states
I am looking forward to an exciting O2k-Workshop, and seeing you next week in Schroecken.
Best regards
Abstract Submission
Abstracts are welcome for short presentations (hot topics in mitochondrial physiology), providing participants for an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.
Lecturers and tutors
- Erich Gnaiger, Organizer, lecturer and tutor; DSL, Medical University Innsbruck, AT; OROBOROS INSTRUMENTS, AT
- Barbara Meissner, Administrative assistant and tutor; OROBOROS INSTRUMENTS, AT
Guest tutors
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