MiPNet23.01 IOC126 Innsbruck AT: Difference between revisions
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{{OROBOROS header page name}} | {{OROBOROS header page name}} | ||
{{Publication | {{Publication | ||
|title= [[Image:OROBOROS-solo.png|right|60px|link= | |title= [[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]] | ||
'''Innsbruck AT''', 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. '''IOC126'''. | '''Innsbruck AT''', 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. '''IOC126'''. | ||
|info= | |info= | ||
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== General information == | == General information == | ||
::::The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric ( | ::::The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (O2k-TPP+ ISE-Module) and fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module). Data analysis - from TPP+ to mt-membrane potential - constitutes a major topic. | ||
[[File:IOC126.png|center|500px|data analysis]] | |||
== Feedback == | |||
::::4 feedback forms returned | |||
{| class="wikitable" | |||
|- | |||
! Question | |||
! Rating (average) | |||
|- | |||
|In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). | |||
|4.50 | |||
|- | |||
|Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). | |||
|2.88 | |||
|- | |||
|Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: | |||
|3.50 | |||
|- | |||
|Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: | |||
|4.25 | |||
|- | |||
|Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). | |||
|3.25 | |||
|- | |||
|Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). | |||
|4.00 | |||
|- | |||
|Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed). | |||
|3.50 | |||
|- | |||
|Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). | |||
|4.00 | |||
|} | |||
== Venue == | == Venue == | ||
::::'''[[IOC Innsbruck]]'''. | :::: Information on travel and venue:'''[[IOC Innsbruck]]'''. | ||
::::» Hotel suggestion: [http://www.weisseskreuz.at/de/index.php Hotel Weisses Kreuz] | ::::» Hotel suggestion: [http://www.weisseskreuz.at/de/index.php Hotel Weisses Kreuz] | ||
:::: Mention "Oroboros" when booking in order to receive a discount. | :::: Mention "Oroboros" when booking in order to receive a discount. | ||
== Programme == | == Programme == | ||
::::{| class='wikitable' | |||
:::: | |- | ||
|style="width: 10em"|'''Wednesday 24th''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Room''' | |||
|- | |||
|09:00 | |||
|Get-together: Introduction of participants and their research interests. | |||
|MiP''Art'' | |||
|- | |||
|09:30 | |||
|Erich Gnaiger: Protonmotive force and respiratory control. [http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21 MitoEAGLE preprint 2017-09-21]. | |||
|MiP''Art'' | |||
|- | |||
|- | |||
|10:00 | |||
|DatLab 7, DL-Protocols. | |||
|MiP''Art'' | |||
|- | |||
|10:30 | |||
|''Coffee/Tea'' | |||
| | |||
|- | |||
|11:00 | |||
|Hands-on: Assembly and maintenance of TPP+ and reference electrodes. [[MiPNet15.03 O2k-MultiSensor-ISE]]. | |||
|MitoFit ORO Lab | |||
|- | |||
|12:15 | |||
|''Lunch break'' | |||
| | |||
|- | |||
|13:30 | |||
|Determination of mt-membrane potential with potentiometric O2k-TPP+ ISE-Module - an introduction. | |||
|MiP''Art'' | |||
|- | |||
|14:30 | |||
|Hands-on: Set up of the instruments with TPP+ and reference electrodes, O2 air calibration and calibration of TPP+ electrode. | |||
|MitoFit ORO Lab | |||
|- | |||
|16:30 | |||
|''Coffee/Tea'' | |||
| | |||
|- | |||
|17:00 | |||
|Hands-on continued: TPP+ chemical background experiment. [[MiPNet14.05 TPP-mtMembranePotential]]. | |||
|MitoFit ORO Lab | |||
|} | |||
::::{| class='wikitable' | |||
|- | |||
|style="width: 10em"|'''Thursday 25th''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Room''' | |||
|- | |||
|09:00 | |||
|Hands-on: O2 air calibration and calibration of TPP+ electrode. | |||
|MitoFit ORO Lab | |||
|- | |||
|09:30 | |||
|Introduction in planning and performing a TPP+ experiment. | |||
|MitoFit ORO Lab | |||
|- | |||
|10:30 | |||
|''Coffee/Tea'' | |||
| | |||
|- | |||
|11:00 | |||
|Hands-on continued: TPP+ experiment with mouse brain mitochondria. | |||
|MitoFit ORO Lab | |||
|- | |||
|12:30 | |||
|''Lunch break'' | |||
| | |||
|- | |||
|14:00 | |||
|From the TPP+ signal to mt-membrane potential - guide through the Excel templates. [[Media:MiPNet14.05_App_TPP-Mathematics-MembranePotential.pdf|TPP-Matematics-MembranePotential]]. | |||
|MitoFit ORO Lab | |||
|- | |||
|14:30 | |||
|Analysis of TPP+ experiment. Interdependence between O2 flux and mt-membrane potential. | |||
|MitoFit ORO Lab | |||
|- | |||
|16:00 | |||
|''Coffee/Tea'' | |||
| | |||
|- | |||
|16:30 | |||
|The four compartment model - the effect of parameters on calculated mt-membrane potential. [http://wiki.oroboros.at/index.php/MiPNet15.03_O2k-MultiSensor-ISE#Calculation_of_mitochondrial_membrane_potential_from_measurement_of_TPP.2B The analysis of the model]. | |||
|MiP''Art'' | |||
|- | |||
|17:30 | |||
|Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. [http://www.mitoeagle.org/index.php/Gnaiger_2017_MiP2017 Abstract]. | |||
|MiP''Art'' | |||
|- | |||
|} | |||
::::{| class='wikitable' | |||
|- | |||
|style="width: 10em"|'''Friday 26th''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Room''' | |||
|- | |||
|09:00 | |||
|Determination of mt-membrane potential with fluorescence method (TMRM, safranin) - O2k FluoRespirometer - an introduction. [[MiPNet22.11 O2k-FluoRespirometer manual]], [[MiPNet20.13 Safranin mt-membranepotential]]. | |||
|MiP''Art'' | |||
|- | |||
|10:00 | |||
|Hands-on: O2 air calibration and O2k setup for fluorescence experiment, choosing fluorescence sensors. | |||
|MitoFit ORO Lab | |||
|- | |||
|10:30 | |||
|''Coffee/Tea'' | |||
| | |||
|- | |||
|11:00 | |||
|Hands-on: Calibration of safranin/TMRM, experiment with mouse brain mitochondria. | |||
|MitoFit ORO Lab | |||
|- | |||
|12:30 | |||
|''Lunch break'' | |||
| | |||
|- | |||
|14:00 | |||
|Analysis of TMRM/safranin experiment, comparison of TPP+ and fluorescence results. | |||
|MitoFit ORO Lab | |||
|- | |||
|15:30 | |||
|''Coffee/Tea'' | |||
| | |||
|- | |||
|16:00 | |||
|Comparison of TPP+ and fluorescence methods for evaluation of mt-membrane potential. Examples in different protocols. | |||
|MiP''Art'' | |||
|- | |||
|17:00 | |||
|Interdependence between O2 flux and mt-membrane potential. | |||
|MiP''Art'' | |||
|- | |||
|17:30 | |||
|The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions. | |||
|MiP''Art'' | |||
|- | |||
|18:00 | |||
|''Farewell activity'' | |||
|MiP''Art'' | |||
|- | |||
|} | |||
== Tutors and Lecturers== | == Tutors and Lecturers== | ||
Line 33: | Line 211: | ||
<br/> | <br/> | ||
<gallery mode=default perrow=6 widths="140px" heights="150px"> | <gallery mode=default perrow=6 widths="140px" heights="150px"> | ||
File:SumbalovaZ.JPG|'''[[Sumbalova Z|Sumbalova | File:SumbalovaZ.JPG|'''[[Sumbalova Z|Zuzana Sumbalova ]]''', PhD, Medical University of Innsbruck - Principal investigator | ||
File:Chang SC.JPG|'''[[Chang SC| | File:Chang SC.JPG|'''[[Chang SC|Shao-Chiang Chang ]]''' MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student [[TRACT]] | ||
File:PassruggerM.JPG|'''[[Passrugger M|Passrugger | File:PassruggerM.JPG|'''[[Passrugger M|Manuela Passrugger ]]''', BSc., Oroboros Instruments - Biomedical Assistant | ||
Image:Bastos Sant'Anna Silva AC.jpg|'''[[Bastos Sant'Anna Silva AC|Ana Carolina Bastos Sant'Anna Silva]]''', MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student [[TRANSMIT]] | Image:Bastos Sant'Anna Silva AC.jpg|'''[[Bastos Sant'Anna Silva AC|Ana Carolina Bastos Sant'Anna Silva]]''', MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student [[TRANSMIT]] | ||
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Erich Gnaiger]]''', Prof., CEO Oroboros Instruments & Medical University of Innsbruck, D. Swarovski Research Laboratory | |||
|- | |- | ||
</gallery> | </gallery> | ||
== List of participants == | == List of participants == | ||
Line 58: | Line 229: | ||
|- | |- | ||
| | | | ||
| [[ | | [[Duicu OM|Duicu Oana-Maria]] | ||
| [[ | | [[RO Timisoara Muntean DM]] - University of Medicine and Pharmacy | ||
|- | |- | ||
| | | | ||
| ''' | | [[de Kok M|de Kok Michéle]] | ||
| ''' | | '''NL Leiden Lindeman JH''' - Leiden University Medical Center | ||
|- | |||
| | |||
| [[Fernandez-Vizarra E|Fernandez-Vizarra Erika]] | |||
| '''UK Cambridge Zeviani M''' - University of Cambridge | |||
|- | |- | ||
| [[File: | | [[File:HungerM.JPG|100px|Hunger Miriam]] | ||
| [[ | | [[Hunger Miriam]] | ||
| [[ | | [[AT Innsbruck Oroboros]] | ||
|- | |- | ||
| | | | ||
Line 73: | Line 248: | ||
| '''NL Leiden Lindeman JH''' - Leiden University Medical Center | | '''NL Leiden Lindeman JH''' - Leiden University Medical Center | ||
|- | |- | ||
| | | [[File:Magda.JPG|70px|Labieniec-Watala Magdalena]] | ||
| [[ | | [[Labieniec-Watala M|Labieniec-Watala Magdalena]] | ||
| | | [[PL Lodz Watala C]] - University of Lodz | ||
|- | |||
| [[File:Michalak S.jpg|100px|Michalak Slawomir]] | |||
| [[Michalak S|Michalak Slawomir]] | |||
| [[PL Poznan Michalak S]] - Poznan University of Medical Sciences | |||
|- | |- | ||
| | | | ||
| [[ | | [[Perez Valencia Juan Alberto]] | ||
| ''' | | '''BR Rio de Janeiro''' - National Cancer Institute | ||
|- | |- | ||
| [[File:SiewieraK.JPG|100px|Siewiera Karolina]] | | [[File:SiewieraK.JPG|100px|Siewiera Karolina]] | ||
Line 85: | Line 264: | ||
| [[PL Lodz Watala C]] - University of Lodz | | [[PL Lodz Watala C]] - University of Lodz | ||
|- | |- | ||
| | | | ||
| | | '''Smirnova Irina''' | ||
| | | '''SE Stockholm Smirnova I''' - Stockholm University | ||
|- | |- | ||
| | | | ||
| [[ | | [[Steffen A|Steffen Anja]] | ||
| ''' | | '''NL Leiden Lindeman JH''' - Leiden University Medical Center | ||
|- | |- | ||
|} | |} | ||
== Registration == | |||
:::: closed | |||
== Impressions from the IOC126== | |||
:::: Pictures taken by: [[Meszaros Andras]] | |||
<gallery mode=default perrow=4 widths="350px" heights="350px"> | |||
File:IOC126-5.jpg|The participants of the IOC126 during a TPP session in the MiPArt | |||
File:IOC126-4.jpg|[[Sumbalova Zuzana|Zuzana Sumbalova ]], one of the tutors of the IOC126 | |||
File:IOC126-7.jpg|Session in the Oroboros MitoFit Laboratory | |||
File:IOC126-8.jpg|The participants [[Michalak Slawomir|Slawomir Michalak ]] and [[Fernandez-Vizarra Erika|Erika Fernandez-Vizarra]] during an experiment | |||
File:IOC126-9.jpg|The tutor [[Chang Shao-Chiang|Shao-Chiang Chang ]] with [[Labieniec-Watala Magdalena|Magdalena Labieniec-Watala]] and [[Perez Valencia Juan Alberto|Juan Alberto Perez Valencia]] | |||
File:IOC-15.jpg|The participants in action | |||
File:IOC126-10.jpg| Tutor [[Passrugger Manuela|Manuela Passrugger]] with [[Steffen Anja|Anja Steffen]] | |||
</gallery> | |||
Line 101: | Line 296: | ||
:::: Oroboros Instruments | :::: Oroboros Instruments | ||
:::: High- | :::: High-resolution respirometry | ||
:::: Schöpfstrasse 18 | :::: Schöpfstrasse 18 | ||
:::: A-6020 Innsbruck, Austria | :::: A-6020 Innsbruck, Austria | ||
Line 112: | Line 307: | ||
::::» '''[[IOC recommended reading]]''' | ::::» '''[[IOC recommended reading]]''' | ||
::::» '''[[MitoPedia: Respiratory states]]''' | ::::» '''[[MitoPedia: Respiratory states]]''' | ||
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link= | :::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | ||
== Project support== | == Project support== | ||
Line 119: | Line 314: | ||
{{Labeling | {{Labeling | ||
|additional=ORO, IOC, MitoFit, | |additional=ORO, IOC, MitoFit, 2018, TRACT, TRANSMIT | ||
}} | }} | ||
Latest revision as of 15:42, 8 June 2020
MiPNet23.01 IOC126 Innsbruck AT
Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126. |
Oroboros (2018-01-24) Mitochondr Physiol Network
Abstract:
Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (O2k-TPP+ ISE-Module) and fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module). Data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
Feedback
- 4 feedback forms returned
Question | Rating (average) |
---|---|
In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). | 4.50 |
Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). | 2.88 |
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: | 3.50 |
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: | 4.25 |
Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). | 3.25 |
Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). | 4.00 |
Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed). | 3.50 |
Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). | 4.00 |
Venue
- Information on travel and venue:IOC Innsbruck.
- » Hotel suggestion: Hotel Weisses Kreuz
- Mention "Oroboros" when booking in order to receive a discount.
Programme
Wednesday 24th Topic Room 09:00 Get-together: Introduction of participants and their research interests. MiPArt 09:30 Erich Gnaiger: Protonmotive force and respiratory control. MitoEAGLE preprint 2017-09-21. MiPArt 10:00 DatLab 7, DL-Protocols. MiPArt 10:30 Coffee/Tea 11:00 Hands-on: Assembly and maintenance of TPP+ and reference electrodes. MiPNet15.03 O2k-MultiSensor-ISE. MitoFit ORO Lab 12:15 Lunch break 13:30 Determination of mt-membrane potential with potentiometric O2k-TPP+ ISE-Module - an introduction. MiPArt 14:30 Hands-on: Set up of the instruments with TPP+ and reference electrodes, O2 air calibration and calibration of TPP+ electrode. MitoFit ORO Lab 16:30 Coffee/Tea 17:00 Hands-on continued: TPP+ chemical background experiment. MiPNet14.05 TPP-mtMembranePotential. MitoFit ORO Lab
Thursday 25th Topic Room 09:00 Hands-on: O2 air calibration and calibration of TPP+ electrode. MitoFit ORO Lab 09:30 Introduction in planning and performing a TPP+ experiment. MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on continued: TPP+ experiment with mouse brain mitochondria. MitoFit ORO Lab 12:30 Lunch break 14:00 From the TPP+ signal to mt-membrane potential - guide through the Excel templates. TPP-Matematics-MembranePotential. MitoFit ORO Lab 14:30 Analysis of TPP+ experiment. Interdependence between O2 flux and mt-membrane potential. MitoFit ORO Lab 16:00 Coffee/Tea 16:30 The four compartment model - the effect of parameters on calculated mt-membrane potential. The analysis of the model. MiPArt 17:30 Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. Abstract. MiPArt
Friday 26th Topic Room 09:00 Determination of mt-membrane potential with fluorescence method (TMRM, safranin) - O2k FluoRespirometer - an introduction. MiPNet22.11 O2k-FluoRespirometer manual, MiPNet20.13 Safranin mt-membranepotential. MiPArt 10:00 Hands-on: O2 air calibration and O2k setup for fluorescence experiment, choosing fluorescence sensors. MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on: Calibration of safranin/TMRM, experiment with mouse brain mitochondria. MitoFit ORO Lab 12:30 Lunch break 14:00 Analysis of TMRM/safranin experiment, comparison of TPP+ and fluorescence results. MitoFit ORO Lab 15:30 Coffee/Tea 16:00 Comparison of TPP+ and fluorescence methods for evaluation of mt-membrane potential. Examples in different protocols. MiPArt 17:00 Interdependence between O2 flux and mt-membrane potential. MiPArt 17:30 The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions. MiPArt 18:00 Farewell activity MiPArt
Tutors and Lecturers
Tutors
Zuzana Sumbalova , PhD, Medical University of Innsbruck - Principal investigator
Shao-Chiang Chang MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRACT
Manuela Passrugger , BSc., Oroboros Instruments - Biomedical Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRANSMIT
Erich Gnaiger, Prof., CEO Oroboros Instruments & Medical University of Innsbruck, D. Swarovski Research Laboratory
List of participants
Participant | Institution | |
---|---|---|
Duicu Oana-Maria | RO Timisoara Muntean DM - University of Medicine and Pharmacy | |
de Kok Michéle | NL Leiden Lindeman JH - Leiden University Medical Center | |
Fernandez-Vizarra Erika | UK Cambridge Zeviani M - University of Cambridge | |
Hunger Miriam | AT Innsbruck Oroboros | |
Janssen Connie | NL Leiden Lindeman JH - Leiden University Medical Center | |
Labieniec-Watala Magdalena | PL Lodz Watala C - University of Lodz | |
Michalak Slawomir | PL Poznan Michalak S - Poznan University of Medical Sciences | |
Perez Valencia Juan Alberto | BR Rio de Janeiro - National Cancer Institute | |
Siewiera Karolina | PL Lodz Watala C - University of Lodz | |
Smirnova Irina | SE Stockholm Smirnova I - Stockholm University | |
Steffen Anja | NL Leiden Lindeman JH - Leiden University Medical Center |
Registration
- closed
Impressions from the IOC126
- Pictures taken by: Meszaros Andras
Zuzana Sumbalova , one of the tutors of the IOC126
The participants Slawomir Michalak and Erika Fernandez-Vizarra during an experiment
The tutor Shao-Chiang Chang with Magdalena Labieniec-Watala and Juan Alberto Perez Valencia
Tutor Manuela Passrugger with Anja Steffen
Contact
- Oroboros Instruments
- High-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Project support
Labels:
ORO, IOC, MitoFit, 2018, TRACT, TRANSMIT
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.