Mitochondrial membrane potential: Difference between revisions
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=== Different methods for measurements of mt-membrane potential === | === Different methods for measurements of mt-membrane potential === | ||
Revision as of 17:13, 29 November 2016
- high-resolution terminology - matching measurements at high-resolution
Mitochondrial membrane potential
Description
The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, Ξpmt.
ΞΟmt = Ξpmt - ΞΒ΅H+ / F
mtMP or ΞΟmt is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per βmotiveβ electron or per electron moved across the transmembrane potential difference, with the number of βmotiveβ electrons expressed in the unit coulomb [C].
The chemical part of the protonmotive force, Β΅H+ / F stems from the difference of pH across the mt-membrane. It contains a factor that bridges the gap between the electric force [J/C] and the chemical force [J/mol]. This factor is the Faraday constant, F, for conversion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol],
F = 96.4853 kJol/V = 96,485.3 C/mol
Abbreviation: mtMP, ΞΟmt
Reference: Mitchell 1961 Nature, Gnaiger 2014 Preface MiP2014
MitoPedia concepts:
Respiratory state,
Recommended
MitoPedia methods:
Respirometry,
Fluorometry
MitoPedia topics:
EAGLE
Communicated by Gnaiger E 2012-10-05, edited 2016-02-06.
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Different methods for measurements of mt-membrane potential
- Mt-membrane potential can either be measured with the O2k-Fluo LED2-Module by using the fluorophores TMRM or Safranin or with the O2k-TPP+ ISE-Module electrode by using the ion reporter TPP+. All mentioned ion reporters inhibit respiration, which makes it essential to test the optimum concentration before starting your experimenal series.
