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| {{Template:OROBOROS support page name}} | | <!-- BANNER ACROSS TOP OF PAGE --> |
| [[File:O2k-support system.jpg|right|350px|Oroboros support system|link=https://wiki.oroboros.at/index.php/O2k-Open_Support]]
| | {{Template:OROBOROS banner}} |
| :::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«</big>
| | {{OROBOROS_support_navigation_line}} |
| | <div id="mp-topbanner" style="clear:both; position:relative; box-sizing:border-box; width:100%; margin:1.2em 0 6px; min-width:47em; border:1px solid #ddd; background-color:#f9f9f9; color:#000; white-space:nowrap;"> |
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| __TOC__
| | <!-- TODAY'S FEATURED CONTENT --> |
| <br /> | | {| role="presentation" id="mp-upper" style="width: 100%; margin-top:4px; border-spacing: 0px;" |
| <br /> | | <!-- 1ST BOX | BIOBLAST ALERT 2023 - LEFT COLUMN --> |
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| | <div id="mp-tfa-h2" style="margin:0.5em; background:#2856ba; font-family:inherit; font-size:120%; font-weight:bold; border:1px solid #a3bfb1; color:#ffffff; padding:0.2em 0.4em;">O2k-Open Support alert - 2024</div> |
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| | {{Template:O2k-OpenSupportAlert2024}} |
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| | <!-- 2ND BOX | SUMMARY - RIGHT COLUMN --> |
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| | <div id="mp-itn" style="padding:1em 1em;">[[File:O2k-support system.jpg|center|350px|Oroboros Ecosystem Agenda ]]</div> |
| | <!-- LEGEND --> |
| | <div style="margin:0.5em; background:#cedff2; font-family:inherit; font-size:120%; font-weight:bold; border:1px solid #a3b0bf; color:#000; padding:0.2em 0.4em;">O2k Support Alert Legend</div> |
| | <div id="mp-itn" style="padding:0.1em 0.6em;">[[File:Footer O2k-OpenSupport-Alert.jpg|center|500px|Oroboros Ecosystem Agenda ]]</div> |
| | </div> |
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| == Apr 2020 ==
| | <!-- STAY ALERT --> |
| * '''Apr-20 Tu'''
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''
| | <div style="margin:0.5em; background:#cedff2; font-family:inherit; font-size:120%; font-weight:bold; border:1px solid #a3b0bf; color:#000; padding:0.2em 0.4em;">Stay alert</div> |
| ::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«
| | <div id="mp-itn" style="padding:0.1em 0.6em;">{{Oroboros Ecosystem Agenda list}}</div> |
| * '''Apr-14 Tu'''
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| | | » [[O2k-OpenSupportAlert2023 Archive]]<br> |
| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?''' | | » [[O2k-OpenSupportAlert2022 Archive]]<br> |
| ::::::: After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]« | | » [[O2k-OpenSupportAlert2021 Archive]]<br> |
| | | » [[O2k-OpenSupportAlert2020 Archive]] |
| * '''Apr-07 Tu'''
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| :::: [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] [[File:Blue-info.png|40px|Info: improve]] '''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?'''' | |
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| ::::::: If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. - »[[SUIT: Browse DL-Protocols and templates]]«
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| * '''Apr-02 Th'''
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| ::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''Do you want to search the best [[SUIT]] protocol for your research questions?''' Use the SUITbrowser: »'''[[SUITbrowser]]'''«
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| == Mar 2020 ==
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| * '''Mar-30 Mo'''
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| ::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:''' »'''[[Enable DL-Protocol editing]]'''«
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| * '''Mar-25 We'''
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]]'''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4: »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«
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| * '''Mar-23 Mo'''
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| ::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module]]?'''
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| ::::::: '''(1) By adjusting the [[Dithionite|dithionite]] concentration to 2.5 mM''', the instrumental O<sub>2</sub> background can be performed with the "BG Feedback" settings. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''«
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| ::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background can be performed in the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''«
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| * '''Mar-11 We'''
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| ::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''
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| ::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«
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| * '''Mar-04 We'''
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| [[File:OroboPOS-Holder002.jpg|right|100px]]
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| ::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''
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| ::::::: See how you can check that the two blue OroboPOS-Holders are intact. - »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«
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| == Feb 2020 ==
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| * '''Feb-28 Fr'''
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| ::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''
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| ::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«
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| == Jan 2020 ==
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| * '''Jan-31 Fr'''
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''
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| ::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »[[Oxygen calibration - DatLab]]«
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| * '''Jan-30 Th'''
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''
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| ::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »[[Oxygen calibration - DatLab]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ‘R1’ and complete the air calibration correctly?'''
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| ::::::: Your air calibration is not effective until you set mark ‘R1’ and click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - »[[Oxygen calibration - DatLab]]«
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| *'''Jan-24 Fr'''
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''
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| ::::::: Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. »[[O2k configuration]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''
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| ::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?
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| ::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]«
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| == Stay alert ==
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| {{Oroboros Ecosystem Agenda list}}
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