O2k-Open Support alert: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{Template:OROBOROS support page name}} | {{Template:OROBOROS support page name}} | ||
[[File:O2k-support system.jpg|right|350px|Oroboros support system|link=https://wiki.oroboros.at/index.php/O2k-Open_Support]] | [[File:O2k-support system.jpg|right|350px|Oroboros support system|link=https://wiki.oroboros.at/index.php/O2k-Open_Support]] | ||
<big>''' | :::::: <big>''Currently Answered Questions, '''CAQ''''', from the Β»[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]Β«</big> | ||
Β | |||
__TOC__ | |||
<br /> | |||
<br /> | <br /> | ||
== Mar 2020 == | == Mar 2020 == | ||
* '''Mar-04 We''' | * '''Mar-04 We''' | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] ''' | [[File:OroboPOS-Holder002.jpg|right|100px]] | ||
::::::: | <br /> | ||
Β | |||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.''' | |||
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - Β»[[O2k-OroboPOS-Holder MitoPedia |O2k-OroboPOS-Holder]]Β« | |||
<br /> | |||
Revision as of 11:28, 5 March 2020
O2k-Open Support alert |
- Currently Answered Questions, CAQ, from the Β»Oroboros Ecosystem AgendaΒ«
Mar 2020
- Mar-04 We
- A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - Β»O2k-OroboPOS-HolderΒ«
- A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
Feb 2020
- Feb-28 Fr
- Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - Β» Oxygen calibration - DatLabΒ«
- Calibration parameters are transferred to experimental DatLab files
Jan 2020
- Jan-31 Fr
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - Β»Oxygen calibration - DatLabΒ«
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- Jan-30 Th
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - Β»Oxygen calibration - DatLabΒ«
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark βR1β and click on Calibrate and copy to clipboard in the O2 calibration window. - Β»Oxygen calibration - DatLabΒ«
- Jan-24 Fr
- Do you write the serial number of the polarographic oxygen sensor (POS) in the βO2k configurationβ window?
- Including the POS serial number in the Β΄O2k configurationΒ΄ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. Β»O2k configurationΒ«
- Do you write the serial number of the polarographic oxygen sensor (POS) in the βO2k configurationβ window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - Β»O2k-Chamber MitoPediaΒ«
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the βO2 calibrationβ window in DatLab?
- The O2 solubility factor varies according to the medium used and influences the air calibration. - Β»Oxygen solubility factorΒ«
- Do you use the correct O2 solubility factor (FM) in the βO2 calibrationβ window in DatLab?
Stay alert
Collections from Bioblast agenda