O2k-Open Support alert: Difference between revisions
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.''' | ||
::::::: Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis - »[[Steady state]]« | ::::::: Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis - »[[Steady state]]« | ||
* '''May-04 Mo''' | |||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you load the correct instrumental background correction accordingly to your range of experimental O<sub>2</sub> levels?''' | |||
::::::: See how to perform the instrumental background test. - »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]« | |||
== Apr 2020 == | == Apr 2020 == | ||
Revision as of 10:07, 29 April 2020
O2k-Open Support alert |
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
May 2020
- May-11 Mo
- Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.
- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis - »Steady state«
- Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.
- May-04 Mo
- Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels?
- See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«
- Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels?
Apr 2020
- Apr-27 Mo
- Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O2 regime?
- To obtain an accurate O2 background correction, you must select the marks that correspond to your range of experimental O2 levels. - » O2 Background correction - troubleshooting«
- Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O2 regime?
- Apr-20 Tu
- Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - » Pyruvate - troubleshooting«
- Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
- Apr-14 Tu
- Why are the oxygen levels different between chambers prior to my experimental run?
- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - » Oxygen calibration - troubleshooting«
- Why are the oxygen levels different between chambers prior to my experimental run?
- Apr-07 Tu
- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - »SUIT: Browse DL-Protocols and templates«
- Apr-02 Th
- Do you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: »SUITbrowser«
Mar 2020
- Mar-30 Mo
- DatLab 7.4 offers the flexibility to edit a DL-Protocol: »Enable DL-Protocol editing«
- Mar-25 We
- O2 flux analysis with DatLab 7.4. Use our latest O2 analysis template DL7.4: »O2k-Videosupport: O2 flux analysis with DatLab 7.4«
- Mar-23 Mo
- New Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
- (1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background can be performed with the "BG Feedback" settings. - »O2k-sV-Module«
- (2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O2 background can be performed in the O2k-sV-Module. - »O2k-sV-Module«
- New Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
- Mar-11 We
- New Do you change the slope smoothing to 20 while running experiments with biological sample?
- While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »Slope smoothing«
- New Do you change the slope smoothing to 20 while running experiments with biological sample?
- Mar-04 We
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
Feb 2020
- Feb-28 Fr
- New Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
- New Calibration parameters are transferred to experimental DatLab files
Jan 2020
- Jan-31 Fr
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »Oxygen calibration - DatLab«
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- Jan-30 Th
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »Oxygen calibration - DatLab«
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark ‘R1’ and click on Calibrate and copy to clipboard in the O2 calibration window. - »Oxygen calibration - DatLab«
- Jan-24 Fr
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. »O2k configuration«
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
- The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
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