SUIT-004: Difference between revisions
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:::: [[SUIT protocol pattern]]: 1PM;2D;3U;4S;5Rot- | :::: [[SUIT protocol pattern]]: 1PM;2D;3U;4S;5Rot- | ||
The SUIT-004 protocols are designed to provide a quick assessment of the linear coupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with NADH-linked substrates ([[PM pathway control state|PM]]) and the contribution of the [[S-pathway control state| S-pathway]] to the ET state ([[NADH_Electron_transfer-pathway_state|N]], [[NS-pathway control state| NS]], [[Succinate pathway control state| S]]). SUIT-004 can be extended with the CIV assay module. | The SUIT-004 protocols are designed to provide a quick assessment of the linear coupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]]) and the contribution of the [[S-pathway control state| S-pathway]] to the ET state ([[NADH_Electron_transfer-pathway_state|N]], [[NS-pathway control state| NS]], [[Succinate pathway control state| S]]). SUIT-004 can be extended with the CIV assay module. | ||
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:::*A succinate concentration of >10 mM may be required for saturating S<sub>''E''</sub> capacity. | :::*A succinate concentration of >10 mM may be required for saturating S<sub>''E''</sub> capacity. | ||
:::+ Linear coupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with N substrates ([[PM pathway control state|PM]]). | :::+ Linear coupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with N substrates ([[PM-pathway control state|PM]]). | ||
:::+ Pathway control in ET state ([[NADH_Electron_transfer-pathway_state|N]], [[NS-pathway control state| NS]], [[Succinate pathway control state| S]]). | :::+ Pathway control in ET state ([[NADH_Electron_transfer-pathway_state|N]], [[NS-pathway control state| NS]], [[Succinate pathway control state| S]]). | ||
:::+ This protocol can be extended with the Complex IV module. | :::+ This protocol can be extended with the Complex IV module. |
Revision as of 14:57, 3 June 2020
Description
Abbreviation: RP1-short
- SUIT protocol pattern: 1PM;2D;3U;4S;5Rot-
The SUIT-004 protocols are designed to provide a quick assessment of the linear coupling control (L- P- E) with NADH-linked substrates (PM) and the contribution of the S-pathway to the ET state (N, NS, S). SUIT-004 can be extended with the CIV assay module.
Communicated by Doerrier C, Iglesias-Gonzalez J, Gnaiger E (last update 2019-06-07)
Specific SUIT protocols
- SUIT-004 O2 pfi D010 for permeabilized fibers
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PM | PML(n) | N | CI | 1PM
|
2D | PMP | N | CI | 1PM;2D
|
2c | PMcP | N | CI | 1PM;2D;2c
|
3U | PME | N | CI | 1PM;2D;3U
|
4S | PMSE | NS | CI&II | 1PM;2D;3U;4S
|
5Rot | SE | S | CII | 1PM;2D;3U;4S;5Rot
|
6Ama | ROX | 1PM;2D;3U;4S;5Rot;6Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- A succinate concentration of >10 mM may be required for saturating SE capacity.
- + Linear coupling control (L- P- E) with N substrates (PM).
- + Pathway control in ET state (N, NS, S).
- + This protocol can be extended with the Complex IV module.
- + Short experimental time.
- - The substrate combination for maximum ET capacity (FNSGP) is not assayed in SUIT-004 in favour of reducing the experimental time.
Compare SUIT protocols
- SUIT-001 (RP1): SUIT-004 is a shortened version of RP1; without G, F and Gp.
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry